Crystal structure of a secreted lipase from Gibberella zeae reveals a novel “double-lock” mechanism  

作　　者：Zhiyong Lou Ming Li Yuna Sun Ye Liu Zheng Liu Wenping Wu Zihe Rao 

机构地区：[1]Laboratory of Structural Biology,Tsinghua University,Beijing 100084,China [2]Novozymes China,14 Xinxi Lu,Shangdi Zone,Haidian District,Beijing 100085,China [3]National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Protein & Cell》2010年第8期760-770,共11页蛋白质与细胞（英文版）

基　　金：supported by the National Programs for High Technology Research and Development Program of China(863 Program)(Grant No.2009ZX10004-304);the National Natural Science Foundation of China(NSFC)(Grant No.30870486);the National Major Project(Grant Nos.2009ZX09311-00,2006AA02A322).

摘　　要：Fusarium graminearum(sexual stage:Gibberella zeae)is the causative agent of Fusarium Head Blight(FHB),which is one of the most destructive plant disease of cereals,accounting for high grain yield losses,especially for wheat and maize.Like other fungal pathogens,several extracellular enzymes secreted by G.zeae are known to be involved in host infection.Among these secreted lipases,G.zeae lipase(GZEL),which is encoded by the FGL1 gene,was demonstrated to be crucial to G.zeae pathogenicity.However,the precise mechanism of GZEL remains unclear due to a lack of detailed structural information.In this study,we report the crystal structure of GZEL at the atomic level.The structure of GZEL displays distinct structural differences compared to reported homologues and indicates a unique“double lock”enzymatic mechanism.To gain insight into substrate/inhibitor recognition,we proposed a model of GZEL in complex with substrate and the lipase inhibitor ebelactone B(based on the reported structures of GZEL homologues),which defines possible substrate binding sites within the catalytic cleft and suggests an“anti sn-l”binding mode.These results pave the way to elucidating the mechanism of GZEL and thus provide clues for the design of anti-FHB inhibitors.

关 键 词：LIPASE Gibberella zeae crystal structure mechanism INHIBITOR 

分 类 号：O62[理学—有机化学]