Quantitative proteomic analysis of S-nitrosated proteins in diabetic mouse liver with ICAT switch method  

作　　者：Xu Zhang Bo Huang Xixi Zhou Chang Chen 

机构地区：[1]National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]Graduate School of the Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Protein & Cell》2010年第7期675-687,共13页蛋白质与细胞（英文版）

基　　金：supported by the National Basic Research Program(973 program)of China(Grant Nos.2006CB911001,2005CB522804);the National Natural Science Foundation of China(Grant Nos.90606020 and 30770512);the Knowledge Innovation Program of the Chinese Academy of Sciences.

摘　　要：In this study we developed a quantitative proteomic method named ICATswitch by introducing isotope-coded affinity tag(ICAT)reagents into the biotin-switch method,and used it to investigate S-nitrosation in the liver of normal control C57BL/6J mice and type 2 diabetic KK-Ay mice.We got fifty-eight S-nitrosated peptides with quantitative information in our research,among which thirty-seven had changed S-nitrosation levels in diabetic mouse liver.The S-nitrosated peptides belonged to fortyeight proteins(twenty-eight were new S-nitrosated proteins),some of which were new targets of S-nitrosation and known to be related with diabetes.S-nitrosation patterns were different between diabetic and normal mice.Gene ontology enrichment results suggested that S-nitrosated proteins are more abundant in amino acid metabolic processes.The network constructed for Snitrosated proteins by text-mining technology provided clues about the relationship between S-nitrosation and type 2 diabetes.Our work provides a new approach for quantifying S-nitrosated proteins and suggests that the integrative functions of S-nitrosation may take part in pathophysiological processes of type 2 diabetes.

关 键 词：ICAT switch mass spectrometry QUANTITATIVE S-NITROSATION type 2 diabetes 

分 类 号：R58[医药卫生—内分泌]