氨基酸残基手性对肽基水凝胶性能的影响  

Effects of the chirality of amino acid residues on the properties of peptide-based hydrogels

作　　者：王天然 李杲[1,2] 贺超良 陈学思[1,2] Tianran Wang;Gao Li;Chaoliang He;Xuesi Chen(Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;School of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]中国科学院长春应用化学研究所,中国科学院生态环境高分子材料重点实验室,长春130022 [2]中国科学技术大学应用化学与工程学院,合肥230026

出　　处：《科学通报》2021年第18期2261-2275,共15页Chinese Science Bulletin

基　　金：国家自然科学基金(51973218,51973220,51833010);中国科学院青年创新促进会资助。

摘　　要：肽基水凝胶作为一种可降解、生物相容性良好的生物材料,其氨基酸残基手性对水凝胶性能具有显著影响.一般情况下,在水凝胶骨架中引入D型氨基酸残基会增强水凝胶对蛋白酶水解的抵抗性,以及增强材料在宿主体内的免疫响应.同时,不同残基手性也会对细胞行为,如干细胞分化、骨修复,以及水凝胶的凝血、抗菌和抗肿瘤性能产生明显的影响.本文综述了近年来氨基酸残基手性在影响肽基水凝胶性能方面的研究,针对开环聚合获得的聚肽和缩合方法(包括固相合成)制备的寡肽与多肽等材料,重点阐述了氨基酸残基手性对肽分子及其水凝胶的二级结构、凝胶化性能、降解、免疫响应等性质,以及体外细胞行为、体内组织再生、抗菌性能和抗肿瘤作用等生物医学应用方面的影响.Hydrogels possess three-dimensional(3 D)hydrophilic or amphiphilic networks composed of a large amount of water.Due to the similarity in physicochemical properties of biological tissues,hydrogels have been widely investigated for various biomedical applications including drug delivery,3 D cell culture,and tissue engineering.Based on the resources,hydrogels can be divided into naturally derived hydrogels and synthetic hydrogels.The former is mainly based on chitosan,hyaluronic acid,gelatin and alginate,while the latter is commonly composed of poly(methacrylic acid)derivatives,polyethers,polyesters,and polypeptides.Among them,synthetic peptide-based hydrogels have attracted widespread attention due to their excellent biocompatibility,biodegradability,and unique compositions and structures analogous to proteins.As the basic structural units of peptide-based hydrogels,amino acids can be divided into L-amino acids and Damino acids according to their chirality,except for achiral glycine.The chirality of amino acid residues will affect the microstructure of the self-assembled constructs of peptides.For example,Schlaad synthesized polypeptides with different secondary structures and properties by homo-or copolymerization ofγ-benzyl glutamate and allylglycine with different chirality.The polypeptides could be made into microspheres by direct emulsification and they showed a spiral surface structure in a dry state.The experiment proved that the spiral structure of the microspheres could be controlled by the chirality of theα-helical polypeptides.The right-handedα-helical polypeptide which is rich in L-amino acids selfassembled into clockwise spiral microsphere,whereas its enantiomer produced counterclockwise spirals.For peptidebased hydrogels,the chirality of amino acid residues affects not only their properties,but also their applications.In this review,we focus on the recent progress in peptide-based hydrogels with different chirality.We summarize the influence of amino acid residues chirality on the secondary structu

关 键 词：肽基水凝胶 聚肽 手性 凝胶性能 生物医学应用 

分 类 号：R318.08[医药卫生—生物医学工程]