A genome-wide RNAi screen identifies genes regulating the formation of P bodies in C. elegans and their functions in NMD and RNAi  

作　　者：Yinyan Sun Peiguo Yang Yuxia Zhang Xin Bao Jun Li Wenru Hou Xiangyu Yao Jinghua Han Hong Zhang 

机构地区：[1]National Institute of Biological Sciences,Beijing 102206,China [2]College of Life Sciences,Beijing Normal University,Beijing 100875,China

出　　处：《Protein & Cell》2011年第11期918-939,共22页蛋白质与细胞（英文版）

基　　金：by the National High Technology Research and Development Program of China(863 Program)(Grant No.2005AA210910).

摘　　要：Cytoplasmic processing bodies,termed P bodies,are involved in diverse post-transcriptional processes including mRNA decay,nonsense-mediated RNA decay(NMD),RNAi,miRNA-mediated translational repression and storage of translationally silenced mRNAs.Regulation of the formation of P bodies in the context of multicellular organisms is poorly understood.Here we describe a systematic RNAi screen in C.elegans that identified 224 genes with diverse cellular functions whose inactivations result in a dramatic increase in the number of P bodies.83 of these genes form a complex functional interaction network regulating NMD.We demonstrate that NMD interfaces with many cellular processes including translation,ubiquitin-mediated protein degradation,intracellular trafficking and cytoskeleton structure.We also uncover an extensive link between translation and RNAi,with different steps in protein synthesis appearing to have distinct effects on RNAi efficiency.Moreover,the intracellular vesicular trafficking network plays an important role in the regulation of RNAi.A subset of genes enhancing P body formation also regulate the formation of stress granules in C.elegans.Our study offers insights into the cellular mechanisms that regulate the formation of P bodies and also provides a framework for system-level understanding of NMD and RNAi in the context of the development of multicellular organisms.

关 键 词：P body stress granules nonsensemediated RNA decay(NMD) RNA interference C.elegans 

分 类 号：R73[医药卫生—肿瘤]