A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture  

作　　者：Qihui Wang Xiaoning Mou Henghua Cao Qingzhang Meng Yanni Ma Pengcheng Han Junjie Jiang Hao Zhang Yue Ma 

机构地区：[1]National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]Graduate School of the Chinese Academy of Sciences,Beijing 100080,China [3]Department of Surgery,Cardiovascular Institute,Fu Wai Heart Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100037,China

出　　处：《Protein & Cell》2012年第1期51-59,共9页蛋白质与细胞（英文版）

基　　金：by the National High Technology Research and Development Program(863 Program;Grant No.2006AA02A106);the National Basic Research Program(973 Program;Grant Nos.2006CB943901,2010CB945024,and 2011CB965002);the Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-YW-R-50);the National Foundation of Science and Technology(No.30640005).

摘　　要：While human induced pluripotent stem cells(hiPSCs)have promising applications in regenerative medicine,most of the hiPSC lines available today are not suitable for clinical applications due to contamination with nonhuman materials,such as sialic acid,and potential pathogens from animal-product-containing cell culture systems.Although several xeno-free cell culture systems have been established recently,their use of human fibroblasts as feeders reduces the clinical potential of hiPSCs due to batch-to-batch variation in the feeders and time-consuming preparation processes.In this study,we have developed a xeno-free and feeder-cell-free human embryonic stem cell(hESC)/hiPSC culture system using human plasma and human placenta extracts.The system maintains the self-renewing capacity and pluripotency of hESCs for more than 40 passages.Human iPSCs were also derived from human dermal fibroblasts using this culture system by overexpressing three transcription factors—Oct4,Sox2 and Nanog.The culture system developed here is inexpensive and suitable for large scale production.

关 键 词：human embryonic stem cells human induced pluripotent stem cells REPROGRAMMING xeno-free and feeder-cell-free culture system 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]