Haploid embryonic stem cells:an ideal tool for mammalian genetic analyses  

作　　者：Linyu Shi Hui Yang Jinsong Li 

机构地区：[1]Group of Epigenetic Reprogramming,State Key Laboratory of Cell Biology,Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Protein & Cell》2012年第11期806-810,共5页蛋白质与细胞（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China(No.2009CB941101 to J.L.);the "Strategic Priority Research Program" of the Chinese Academy of Sciences(No.XDA01010403 to J.L.).

摘　　要：Identification of the function of all genes in the mammalian genome is critical in understanding basic mechanisms of biology.However,the diploidy of mammalian somatic cells has greatly hindered efforts to elucidate the gene function in numerous biological processes by mutagenesis-based genetic approaches.Recently,mouse haploid embryonic stem(haES)cells have been successfully isolated from parthenogenetic and androgenetic embryos,providing an ideal tool for genetic analyses.In these studies,mouse haES cells have already shown that they could be used in cell-based forward or reverse genetic screenings and in generating gene-targeting via homologous recombination.In particular,haES cells from androgenetic embryos can be employed as novel,renewable form of fertilization agent for yielding live-born mice via injection into oocytes,thus showing the possibility that genetic analysis can be extended from cellular level to organism level.

关 键 词：haploid embryonic stem cells parthenogenetic embryos androgenetic embryos genetic screening DIPLOID genomic imprinting 

分 类 号：R73[医药卫生—肿瘤]