Cell type specificity of signaling:view from membrane receptors distribution and their downstream transduction networks  

作　　者：Ying He Zhonghao Yu Dongya Ge Rui Wang-Sattler Hans-Jurgen Thiesen Lu Xie Yixue Li 

机构地区：[1]Key Laboratory of Systems Biology,Chinese Academy of Sciences,Shanghai 200031,China [2]Shanghai Center for Bioinformation Technology,Shanghai 200235,China [3]Graduate University of the Chinese Academy of Sciences,Shanghai 200031,China [4]Research unit of Molecular Epidemiology,Helmholtz Zentrum Munchen,Neuherberg 85764,Germany [5]Office of Technology Transfer,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China [6]Institut fur Immunologie,Universitat Rostock,Rostock 18057,Germany

出　　处：《Protein & Cell》2012年第9期701-713,共13页蛋白质与细胞（英文版）

基　　金：kindly funded by National Natural Science Foundation of China(Grant No.31070752);in part supported by the National Basic Research Program(973 Program)(Nos 2011CB910204,2010CB529206 and 2010CB912702);Key Infectious Disease Project(No.2012ZX10002012-014);Research Program of Chinese Academy of Sciences(Nos.KSCX2-EW-R-04,KSCX2-YW-R-190 and 2011KIP204);National Natural Science Foundation of China(Grant No.30900272);Chinese Ministry for Science and Technology Grant(No.2008BAI64B01);the National High Technology Research and Development Program(863 Program)(No.2009AA02Z304).

摘　　要：Studies on cell signaling pay more attention to spatial dynamics and how such diverse organization can relate to high order of cellular capabilities.To overview the specificity of cell signaling,we integrated human receptome data with proteome spatial expression profiles to systematically investigate the specificity of receptors and receptor-triggered transduction networks across 62 normal cell types and 14 cancer types.Six percent receptors showed cell-type-specific expression,and 4% signaling networks presented enriched cell-specific proteins induced by the receptors.We introduced a concept of“response context”to annotate the cell-type dependent signaling networks.We found that most cells respond similarly to the same stimulus,as the“response contexts”presented high functional similarity.Despite this,the subtle spatial diversity can be observed from the difference in network architectures.The architecture of the signaling networks in nerve cells displayed less completeness than that in glandular cells,which indicated cellular-context dependent signaling patterns are elaborately spatially organized.Likewise,in cancer cells most signaling networks were generally dysfunctional and less complete than that in normal cells.However,glioma emerged hyper-activated transduction mechanism in malignant state.Receptor ATP6AP2 and TNFRSF21 induced rennin-angiotensin and apoptosis signaling were found likely to explain the glioma-specific mechanism.This work represents an effort to decipher context-specific signaling network from spatial dimension.Our results indicated that although a majority of cells engage general signaling response with subtle differences,the spatial dynamics of cell signaling can not only deepen our insights into different signaling mechanisms,but also help understand cell signaling in disease.

关 键 词：plasma membrane receptor cellular signaling transduction network diversity cell type specific spatial expression profile 

分 类 号：R73[医药卫生—肿瘤]