Immune modulatory function of abundant immune-related microRNAs in microvesicles from bovine colostrum  

作　　者：Qi Sun Xi Chen Jianxiong Yu Ke Zen Chen-Yu Zhang Liang Li 

机构地区：[1]Jiangsu Engineering Research Center for microRNA Biology and Biotechnology,State Key Laboratory of Pharmaceutical Biotechnology,Nanjing University School of Life Sciences,Nanjing 210093,China [2]Department of Virology,University of California School of Public Health,Berkeley,CA 94720,USA

出　　处：《Protein & Cell》2013年第3期197-210,共14页蛋白质与细胞（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(Grant Nos.30988003,31071232,3100032331000478 and 90608010);the National Basic Research Program(973 Program)(No.2011CB504803).

摘　　要：Colostrum provides essential nutrients and immunologically active factors that are beneficial to newborns.Our previous work demonstrated that milk contains large amounts of miRNA that is largely stored in milk-derived microvesicles(MVs).In the present study,we found that the MVs from colostrum contain signifi cantly higher levels of several immune-related miRNAs.We hypothesized that the colostrum MVs may transfer the immune-related miR-NAs into cells,which contribute to its immune modulatory feature.We isolated colostrum MVs by ultracentrifugation and demonstrated several immune modulation features associated with miRNAs.We also provide evidence that the physical structure of milk-derived MVs is essential for transfer miRNAs and following immune modulation effect.Moreover,we found that colostrum powder-derived MVs also contains higher levels of immune-related miRNAs that display similar immune modulation effects.Taken together,these results show that MV-containing immunerelated miRNAs may be a novel mechanism by which co-lostrum modulates body immune response.

关 键 词：COLOSTRUM MIRNAS MICROVESICLES immune modulation 

分 类 号：S82[农业科学—畜牧学]