SIX1、E-cad在子宫内膜增生性病变组织中的表达及意义  

The expression and significance of SIX1 and E-cad levels in pathological tissue of endometrial hyperplasia

作　　者：朱惠萍 王丽丹 ZHU Huiping;WANG Lidan(Zhuji People's Hospital,Zhejiang Province,311800)

机构地区：[1]浙江省诸暨市人民医院,311800

出　　处：《中国计划生育学杂志》2021年第6期1154-1157,1162,1309,共6页Chinese Journal of Family Planning

摘　　要：目的:探究单纯性增生患者、复杂性增生伴非典型增生患者内膜组织中同源盒基因SIX1、上皮型黏附素(E-cad)mRNA和蛋白表达及意义。方法:选取2019年12月-2021年3月于本院就诊并行刮宫术的159例子宫内膜增生性病变患者的病变内膜组织标本,其中单纯性增生(单纯增生组)81例,复杂性增生伴非典型增生(复杂增生组)78例;选取同期子宫内膜癌患者83例正常子宫内膜组织作为内膜癌组。采用实时荧光定量PCR(qRT-PCR)法检测3组内膜组织SIX1、E-cad mRNA水平,免疫组织化学法检测内膜组织中SIX1、E-cad蛋白表达;采用Pearson法、Spearman法分析子宫内膜增生性病变患者SIX1与E-cad mRNA水平及蛋白表达相关性。结果:复杂性增生组SIX1 mRNA水平及蛋白表达阳性率最高、单纯增生组次之、内膜癌组最低,E-cad mRNA水平及蛋白表达阳性率最低、单纯增生组次之、内膜癌组(P<0.05);单纯增生组SIX1 mRNA水平及蛋白表达阳性率高于内膜癌组,E-cad mRNA水平及蛋白表达阳性率低于内膜癌组(P<0.05)。单纯性增生及复杂性增生伴非典型增生患者SIX1与E-cad mRNA水平均呈负相关(r=-0.664、-0.572,P<0.05),SIX1与E-cad蛋白表达均呈负相关(r=-0.354、-0.424,P<0.05)。结论:子宫内膜增生性病变患者内膜组织中SIX1表达水平升高,E-cad表达水平降低,其异常表达可能与子宫内膜增生的发病有关。Objective: To explore the expression and significance of mRNA and protein of sine oculis homeobox homolog 1(SIX1) and E-cadherin(E-cad) in the endometrial tissue of patients with simple endometrial hyperplasia, with complex endometrial hyperplasia, or with atypical endometrial hyperplasia. Methods: The diseased endometrial tissues were collected from 159 patients with endometrial hyperplasia after curettage as the research objects from December 2019 to March 2021. Among these patients, there were 81 cases with simple endometrial hyperplasia in group A and 78 cases with complex and atypical endometrial hyperplasia in group B. The normal endometrial tissues of 83 patients with endometrial cancer were selected in group C during the same period. Real-time fluorescence quantitative PCR(qRT-PCR) method was used to detect the mRNA levels of SIX1 and E-cad mRNA in endometrial tissues of the patients in the three groups, and immunohistochemical method was used to detect their expression of SIX1 and E-cad proteins in endometrial tissues. Pearson’s method and Spearman’s method were used to analyze the correlation between SIX1 and E-cad mRNA levels of the patients with endometrial hyperplasia and their protein expression. Results: The SIX1 mRNA level and the positive rate of protein expression of the patients in group B were the highest, followed by those of the patients in group A, and which of the patients in group C were the lowest. The E-cad mRNA level and the positive rate of protein expression of the patients in group B were the lowest, followed by those of the patients in group A, and which of the patients in group C were the highest. The level of SIX1 mRNA and the positive rate of protein expression of the patients in group A were significant higher than those of the patients in group C, and the level of E-cad mRNA and the positive rate of protein expression of the patients in group A were significant lower than those of the patients in group C(P<0.05).In group A and B,the SIX1 level was negatively correlation wi

关 键 词：子宫内膜 单纯性增生 复杂性增生伴非典型增生 同源盒基因SIX1 上皮型黏附素 

分 类 号：R737.33[医药卫生—肿瘤]