乳腺癌组织中miR-502和HDAC3表达的相关性及其临床意义  

Correlation of miR-502 and HDAC3 expression in breast cancer tissue and its clinic significance

作　　者：赵云飞[1] 何姣[1] 杨玲[1] 刘智[1] 罗启翅 陈茂山[2] ZHAO Yunfei;HE Jiao;YANG Ling;LIU Zhi;LUO Qichi;CHEN Maoshan(Department of Pathology,Surgery,Suining Central Hospital,Suining,Sichuan 629000,China;Department of Breast and Thyroid,Surgery,Suining Central Hospital,Suining,Sichuan 629000,China)

机构地区：[1]遂宁市中心医院病理科,四川省遂宁市629000 [2]遂宁市中心医院乳腺甲状腺外科,四川省遂宁市629000

出　　处：《中南医学科学杂志》2021年第4期447-451,共5页Medical Science Journal of Central South China

基　　金：四川省卫生和计划生育委员会科研课题(17PJ599);遂宁市中心医院科研项目(2015y03)。

摘　　要：目的探讨miR-502和HDAC3在乳腺癌中的表达关系及其临床病理意义。方法采集76例乳腺癌患者癌旁组织及癌组织标本,实时荧光定量PCR检测组织中miR-502、HDAC3 mRNA的表达,并绘制溶解曲线。免疫组化及免疫印迹法检测各组织标本中HDAC3阳性表达率及蛋白表达,并对乳腺癌组织中miR-502与HDAC3的相关性及靶向关系进行分析验证。分析miR-502、HDAC3表达水平与乳腺癌患者临床病理特征、诊断效能及生存情况之间的关系。结果乳腺癌组织中miR-502呈低表达水平,而HDAC3呈高表达水平(P<0.05),且二者水平在乳腺癌组织中呈负相关(P<0.05),HDAC3是miR-502的靶基因。miR-502、HDAC3相对表达水平与TNM分期、肿瘤直径、淋巴结转移及雌激素受体/孕激素受体表达情况有关(P<0.05)。乳腺癌组织中miR-502、HDAC3二者联合诊断效能高于miR-502、HDAC3单一诊断效能。miR-502高表达组患者3年总生存率高于低表达组(P<0.05);HDAC3高表达组患者3年总生存率低于低表达组(P<0.05)。结论miR-502、HDAC3在乳腺癌中的表达与患者病情发展及预后相关,可作为早期诊断乳腺癌的生物学指标。Aim To explore the relationship between miR-502 and HDAC3 expression in breast cancer and its clinicopathological significance.Methods The paracancerous tissues and cancer tissue samples of 76 breast cancer patients were collected,Real-time quantitative fluorescence PCR was used to detect and plot the mRNA expressions of miR-502 and HDAC3 in the tissues,and the dissolution curves were drawn.The positive expression rate and protein expression of HDAC3 were detected by immunohistochemistry and western blot,the correlation and targeting relationship between miR-502 and HDAC3 in breast cancer tissues were analyzed and verified,and to investigate the relationship between miR-502 and HDAC3 expression levels and clinicopathological characteristics,diagnostic efficacy and survival of breast cancer patients.Results miR-502 level was low in breast cancer tissues,while HDAC3 level was high(P<0.05),and the two levels were negatively correlated in breast cancer tissues(P<0.05),and HDAC3 was the target gene of miR-502.The relative expression levels of miR-502 and HDAC3 were correlated with TNM stage,tumor diameter,lymph node metastasis and estrogen receptor/progesterone receptor expression(P<0.05).The combined diagnostic efficacy of miR-502 and HDAC3 was higher than that of miR-502 and HDAC3 alone in breast cancer tissue.The 3-year overall survival rate in the group with high miR-502 expression was higher than that in the group with low expression(P<0.05),the 3-year overall survival rate in the group with high HDAC3 expression was significantly lower than that in the group with low expression(P<0.05).Conclusion The expression of miR-502 and HDAC3 in breast cancer is correlated with the disease progression and prognosis of patients,and can be used as a biological indicator for early diagnosis of breast cancer.

关 键 词：miR-502 HDAC3 乳腺癌 表达关系 生存分析 诊断效能 

分 类 号：R737.9[医药卫生—肿瘤]