HPV16感染与宫颈鳞癌的相关性及对PI3K/AKT信号通路表达水平的影响  

Correlation between HPV16 infection and cervical squamous cell carcinoma and its effect on the PI3K/AKT signaling pathway

作　　者：李柱娟 李华 陈丽 LI Zhu-juan;LI Hua;CHEN Li(Department of Gynecology,Jiangjin District Central Hospital,Chongqing 402360,China)

机构地区：[1]重庆市江津区中心医院妇科,重庆402360

出　　处：《热带医学杂志》2021年第5期618-622,共5页Journal of Tropical Medicine

基　　金：重庆市科卫联合医学科研项目(2018MSXM098)。

摘　　要：目的探究人乳头瘤病毒(HPV)16感染与宫颈鳞癌的相关性及对磷酯酰肌醇-3激酶/蛋白激酶B(PI3K/AKT)信号通路表达水平的影响。方法2015年6月至2019年1月重庆市江津区中心医院收治的宫颈病变患者100例作为研究对象,检测患者的HPV16感染情况,采用免疫组织化学法比较不同宫颈病变患者的PI3K、AKT、NF-κB、P27蛋白激酶抑制物1(p27kip1)表达情况。结果宫颈鳞癌患者的HPV16感染率82.22%显著高于慢性子宫颈炎的5.56%及宫颈上皮内瘤样病变(CIN)的48.65%,差异有统计学意义(P<0.05);宫颈鳞癌分化程度高、临床分期为Ⅲa+Ⅲb的患者HPV16感染率显著高于低+中分化及Ⅰa+Ⅰb分期(P<0.05);宫颈鳞癌患者的PI3K、AKT表达阳性率(73.33%、80.00%)显著高于慢性子宫颈炎的(分别为27.78%、22.22%)及CIN的(分别为56.76%、54.05%),差异有统计学意义(P<0.05)。宫颈鳞癌患者的NF-κB表达阳性率(71.11%)显著高于慢性子宫颈炎(5.56%)及CIN(64.86%),p27kip1表达阳性率(33.33%)显著低于慢性子宫颈炎(94.44%)及CIN(70.27%),差异有统计学意义(P<0.05);不同宫颈鳞癌分化程度及临床分期患者的PI3K、AKT、NF-κB、p27kip1表达阳性率差异有统计学意义(P<0.05)。Pearson相关分析结果显示,宫颈鳞癌的分化程度及临床分期与PI3K、AKT、NF-κB的表达呈正相关(r=0.291、0.527、0.449,P<0.05),与p27kip1表达呈负相关(r=-0.326,P<0.05)。HPV16感染与PI3K、AKT、NF-κB表达在子宫颈病变过程中呈正相关(r=0.315、0.241、0.657,P<0.05),与p27kip1表达呈负相关(r=-0.373,P<0.05)。结论HPV16感染可能影响PI3K/AKT信号通路分子表达,在子宫颈上皮慢性炎症-CIN-宫颈癌的病情发展中发挥重要作用,为宫颈鳞癌的临床治疗提供新思路。Objective To explore the correlation between human papilloma virus(HPV)16 infection and cervical squamous cell carcinoma and its effect on the phosphatidyl alcohol-3 kinase/protein kinase B(PI3 K/AKT)signaling pathway.Methods From June 2015 to January 2019,100 patients with cervical lesions admitted to our hospital were included in the study.Immunohistochemistry methods were used to detect HPV16 infection in patients,and to compare the expression of PI3 K,AKT,NF-κB and P27 protein kinase inhibitor(p27 kip1)in patients with different cervical lesions.Results The HPV16 infection rate in patients with cervical squamous cell carcinoma was significantly higher than that of chronic cervicitis(5.56%)and cervical intraepithelial neoplasia(CIN)(48.65%)(P<0.05).The HPV16 infection rate of patients with high degree of differentiation of cervical squamous cell carcinoma and clinical stageⅢa+Ⅲb was significantly higher than that of low+middle differentiation andⅠa+Ⅰb stage(P<0.05).The positive rates of PI3 K and AKT expression in patients with cervical squamous cell carcinoma(73.33%,80.00%)were significantly higher than those of chronic cervicitis(27.78%,22.22%)and CIN(56.76%,54.05%)(P<0.05).The positive rate of NF-κB expression in cervical squamous cell carcinoma patients(71.11%)was significantly higher than those in chronic cervicitis(5.56%)and CIN(64.86%),and the positive rate of p27 kip1 expression(33.33%)was significantly lower than those in chronic cervicitis(94.44%)and CIN(70.27%)(P<0.05).The positive rates of PI3 K,AKT,NF-κB and p27 kip1 expression in different degrees of cervical squamous cell carcinoma differentiation and clinical stage were significantly different(P<0.05).Pearson correlation analysis showed that cervical squamous cell carcinoma differentiation and clinical stage and PI3 K,the expressions of AKT and NF-κB were positively correlated(r=0.291,0.527,0.449,P<0.05),and negatively correlated with p27 kip1 expression(r=-0.326,P<0.05).HPV16 infection was positively correlated with PI3 K,AKT and N

关 键 词：人乳头状瘤病毒16 PI3K/AKT信号通路 宫颈病变 鳞状细胞癌 

分 类 号：R737.33[医药卫生—肿瘤]