雌二醇对原代人牙周膜细胞生物学特性的影响  

Estradiol affects the biological characteristics of human periodontal ligament cells in vitro

作　　者：赵迎帆 薛楠[1] 张扬[1] ZHAO Ying-fan;XUE Nan;ZHANG Yang(Department of Orthodontics,School and Hospital of Stomatology,China Medical University,Liaoning Provincial Key Laboratory of Oral Diseases,Shenyang 110002,China)

机构地区：[1]中国医科大学口腔医学院附属口腔医院正畸一科,辽宁省口腔疾病重点实验室,辽宁沈阳110002

出　　处：《解剖科学进展》2021年第3期301-304,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(2020-BS-121)。

摘　　要：目的探讨不同浓度17β-雌二醇对原代人牙周膜细胞(human periodontal ligament cells,hPDLCs)增殖、细胞周期以及碱性磷酸酶活性的影响。方法无菌条件下刮取人牙周膜组织,采用组织块结合酶消化法进行原代培养并用免疫组化法鉴定。处于对数生长期的第4代hPDLCs分别加入不同浓度的17β-雌二醇(10^(-5)、10^(-6)、10^(-7)、10^(-8)、10^(-9)mol/L),培养24h、48h、72h和7d后,MTS比色法检测17β-雌二醇对hPDLCs细胞增殖的影响;流式细胞术检测hPDLCs细胞周期的变化;BCIP/NBT法检测17β-雌二醇对hPDLCs碱性磷酸酶活性的影响。结果分离培养的原代hPDLCs中Vimentin呈阳性表达,而Cytokerain呈阴性表达,证实hPDLCs分离培养成功。17β-雌二醇能促进hPDLCs增殖,调控细胞周期进程并提高碱性磷酸酶活性,其中以10^(-8)mol/L浓度的作用最为显著。结论一定浓度范围内的17β-雌二醇对hPDLCs增殖、分化有一定的诱导作用。Objective To investigate the effect of 17β-estradiol on the proliferation, cell cycle progression and alkalinephosphatase(ALP) activity of human periodontal ligament cells(h PDLCs). Methods The primary h PDLCs were isolated, the dental periodontal ligament tissues were collected under sterile condition and treated with Type I collagenase for cell dispersion. Immunohistochemical staining of Vimentin and Cytokerain was performed for the identification of h PDLCs which were incubated with various concentrations of 17β-estradiol(10^(-5)、10^(-6)、10^(-7)、10^(-8)、and 10^(-9)mol/L)for 24 h, 48 h, 72 h, and 7 d. The proliferation of h PDLCs was detected by MTS assay. Flow cytometry was used for evaluating cell cycle progression. The activity of ALP was assessed by BCIP-NBT assay. Results Vimentin expression was positive, but negative for Cytokerain expression in the isolated h PDLCs. 17β-estradiol promoted the proliferation, cell cycle progression, and enhanced ALP activity of h PDLCs with optimal concentration of 10^(-8) mol/L. Conclusion 17β-estradiol promoted the growth and osteogenic differentiation of h PDLCs.

关 键 词：17Β-雌二醇 原代人牙周膜细胞 细胞增殖 细胞周期 碱性磷酸酶 

分 类 号：R782.2[医药卫生—口腔医学]