ARID1A及PIK3CA在膀胱尿路上皮癌中的表达及临床病理意义  

Expression and significance of ARID1A and PIK3CA in bladder urothelial carcinoma

作　　者：王晨静[1] 李超[2] 咸娴 王胜[2] 何雷 范多娇 WANG Chenjing;LI Chao;XIAN Xian;WANG Sheng;HE Lei;FAN Duojiao(Department of Pathology,Baoding Second Hospital,Baoding,Hebei,071051,China;Department of Pathology,Dingzhou People's Hospital;Department of Urology,Baoding Second Hospital;General Surgery and Oncology Department,Xushui District Peoples Hospital;Department of Science and Education,Baoding Second Hospital)

机构地区：[1]保定市第二医院病理科,河北保定071051 [2]定州市人民医院病理科 [3]保定市第二医院泌尿外科 [4]徐水区人民医院普外肿瘤科 [5]保定市第二医院科教科

出　　处：《临床泌尿外科杂志》2021年第6期425-430,共6页Journal of Clinical Urology

基　　金：保定市科学技术研究与发展计划项目(No:2041ZF021)。

摘　　要：目的:探讨膀胱尿路上皮癌(BUC)组织中ARID1A及PIK3CA的表达及意义。方法:采用免疫组化EnVision法检测107例膀胱尿路上皮癌(BUC组)及28例癌旁组织(癌旁组)中ARID1A、PIK3CA及Ki-67的表达水平,并将BUC组按照临床病理特征进行分组,统计分析ARID1A、PIK3CA在各组间表达的差异及相关性。结果:ARID1A在BUC组中有42.06%呈缺失表达,而在癌旁组中仅7.14%呈缺失表达,二者差异有统计学意义(P<0.05)。PIK3CA在BUC组中有74例(69.16%)表达显著增强,而在癌旁组中仅7例(25%)呈强阳性表达,两组间比较差异有统计学意义(P<0.05)。结合患者的临床病理特征进行分析,发现ARID1A的缺失表达与肿瘤的浸润深度、分级及Ki-67增殖指数有关(P<0.05),PIK3CA的阳性表达与肿瘤的浸润深度有关(P<0.05),与分级、Ki-67增殖指数无关(P>0.05)。相关性分析结果显示ARID1A与PIK3CA之间的表达无明确相关性(r=-0.062,P>0.05),复发与否与年龄、病变级别、浸润深度、PIK3CA蛋白阳性表达率呈正相关。结论:ARID1A及PIK3CA在BUC患者癌组织中的表达明显增强,且与病变级别、肿瘤浸润密切相关,但两种蛋白间并无相关性,二者可能通过不同的途径参与BUC的发生、进展。并且PIK3CA蛋白阳性表达可明显增加BUC的复发率,进一步为探究BUC提供了新的思路。Objective:To investigate the expression and significance of ARID1 A and PIK3 CA in bladder urothelial carcinoma(BUC).Methods:Immunohistochemical methods were employed to evaluate protein level of ARID1 A and PIK3 CA in 107 cases of BUC and 28 cases of adjacent tissues.The BUC tissues were grouped by clinical and pathological features,and the difference and relations of ARID1 A and PIK3 CA in each group were analyzed.Results:The loss rate of ARID1 A was 42.06%in BUC,but only 7.14%in adjacent tissues.The positive rate of PIK3 CA in BUC(69.16%)was significantly higher than that in adjacent tissues(25%)(P<0.05).The clinical features of 107 cases of BUC were retrospectively analyzed,which showed that the expression of ARID1 A was significantly associated with histopathology grading,depth of invasion and Ki-67 index.The expression of PIK3 CA was significantly associated with depth of invasion,but not related to age,sexuality,recurrence or Ki-67 index(P>0.05).The results of correlation analysis showed that there was no clear correlation between ARID1 A and PIK3 CA(r=-0.062,P>0.05).The recurrence was positively correlated with age,histopathology grading,depth of invasion and PIK3 CA.Conclusion:ARID1 A and PIK3 CA play an important role in the development and progression of BUC through different pathways.The expression of PIK3 CA maybe increase the recurrence of BUC.It will provide a new idea for exploring the prevention and treatment of urothelial carcinoma of the bladder.

关 键 词：膀胱肿瘤 尿路上皮癌 AT丰富结合域1A 磷脂酰肌醇3-激酶催化亚基 免疫组织化学 

分 类 号：R737.11[医药卫生—肿瘤]