调节性T细胞在前列腺癌患者外周血中表达的研究  

Expression of regulatory T cells in peripheral blood of prostate cancer patients

作　　者：李宁[1] 董彤 王于勇[1] 胡逸轩 李昶灸 谢玺 何华东 LI Ning;DONG Tong;WANG Yuyong;HU Yixuan;LI Changjiu;XIE Xi;HE Huadong(Department of Urology,Affiliated Hangzhou First People's Hospital,School of Medicine,Zhejiang University,Hangzhou,310006,China;Affiliated Hangzhou Hospital,Nanjing Medical University)

机构地区：[1]浙江大学医学院附属杭州市第一人民医院泌尿外科,杭州310006 [2]南京医科大学附属杭州医院

出　　处：《临床泌尿外科杂志》2021年第6期473-477,共5页Journal of Clinical Urology

基　　金：浙江省医药卫生科技青年人才计划项目(No:2019RC241)。

摘　　要：目的:探讨不同分期前列腺癌患者外周血中CD4^(+)CD25^(+)CD127^(low/-)调节性T细胞(Tregs)比例变化及临床意义。方法:将2018年5月—2019年11月期间杭州市第一人民医院泌尿外科收治的部分前列腺癌患者(共90例)进行分组并招募志愿者,采集外周血处理后行流式细胞术,分析前列腺癌患者与正常对照组,以及不同分级前列腺癌组CD4^(+)CD25^(+)CD127^(low/-)表达水平是否存在差异性。结果:前列腺癌患者组外周血中的CD4^(+)CD25^(+)CD127^(low/-)Tregs表达水平与对照组比较差异有统计学意义(P<0.01);随着危险分级升高CD4^(+)CD25^(+)CD127^(low/-)Tregs的表达水平有升高趋势,且不同分级间表达水平差异有统计学意义(高危组vs.中危组,P<0.05;高危组vs.低危组,P<0.001;中危组vs低危组,P<0.001)。结论:外周血中CD4^(+)CD25^(+)CD127^(low/-)Tregs在前列腺癌患者中显著增加,且与前列腺癌的发生发展密切相关,其可为前列腺癌患者早期诊断和免疫治疗提供理论基础。Objective:To explore the changes and clinical significance of CD4^(+)CD25^(+)CD127^(low/-)regulatory T cells(Tregs)in peripheral blood of patients with prostate cancer(PCa)at different stages.Methods:We selected some patients with PCa admitted from May 2018 to Nov.2019 and recruited volunteers,and collected their peripheral blood for flow cytometry.The expression of CD4^(+)CD25^(+)CD127^(low/-)Tregs in PCa patients of different grades and volunteers groups were analysed.Results:The expression of CD4^(+)CD25^(+)CD127^(low/-)Tregs in the peripheral blood of patients with PCa was significantly different from that of the volunteers group(P<0.01).With the increase of grade,the expression of CD4^(+)CD25^(+)CD127^(low/-)Tregs increased,and the difference in expression levels among different grades was statistically significant.Conclusion:CD4^(+)CD25^(+)CD127^(low/-)Tregs in peripheral blood significantly increases in patients with PCa,and is closely related to the occurrence and development of PCa,which can provide a theoretical basis for early diagnosis and immunotherapy of patients with PCa.

关 键 词：前列腺癌 外周血 免疫治疗 

分 类 号：R737.25[医药卫生—肿瘤]