慢性咽炎患者咽分泌物中幽门螺杆菌的超高倍显微镜检测结果  

Detection of Helicobacter pylori in pharyngeal secretion of patients with chronic pharyngitis by ultra high power microscope

作　　者：舒静 张晓方 倪慧 张静 Shu Jing;Zhang Xiaofang;Ni Hui;Zhang Jing(Department of laboratory,Dongchangfu maternal and child health hospital,Liaocheng,Shandong,252000,China)

机构地区：[1]聊城市东昌府区妇幼保健院,山东聊城252000

出　　处：《中国医学文摘（耳鼻咽喉科学）》2021年第3期56-59,共4页Chinese Medical Digest(Otorhinolaryngology)

摘　　要：目的慢性咽炎患者咽分泌物中幽门螺杆菌(HP)的超高倍显微镜检测结果。方法用2000倍超高倍显微镜对300例慢性咽炎患者和40例正常人进行了观察。根据观测需要,选择暗场差分场进行现场观测,由专家确定观测结果。采用卡方检验评价两组Hp阳性率的差异。结果300例慢性咽炎组233例咽部分泌物中检出HP感染,阳性率为77.67%(233/300),阴性67例,阴性率为22.33%(67/300)。正常对照组仅检出2例HPi感染,阳性率为5.00%(2/40),阴性38例,阴性率为95.00%(38/40)。慢性咽炎患者咽部分泌物Hp检出率与正常对照组比较差异有统计学意义(P<0.05)。结论幽门螺杆菌可能是慢性咽炎患者忽视喉部的原因,超高功率显微镜可作为咽部分泌物螺旋菌筛选的新方法,幽门螺杆菌感染与喉返流的关系有待进一步研究。Objective To detect Helicobacter pylori(HP)in pharyngeal secretions of patients with chronic pharyngitis by ultrahigh power microscope.Methods 300 cases of chronic pharyngitis and 40 normal people were observed by 2000 times ultrahigh magnification microscope.According to the observation needs,dark field difference field is selected for on-site observation,and the observation results are determined by experts.Chi-square test was used to evaluate the difference of Hp positive rate between the two groups.Results Hp infection was detected in 233 cases of pharyngeal secretions in 300 cases of chronic pharyngitis group,with a positive rate of 77.67%(233/300)and a negative rate of 67 cases(22.33%,67/300).In normal control group,only 2 cases of HPI infection were detected,the positive rate was 5.00%(2/40),and 38 cases were negative,the negative rate was 95.00%(38/40).The detection rate of Hp in the pharyngeal secretions of patients with chronic pharyngitis was significantly different from that of normal control group(P<0.05).Conclusion Helicobacter pylori may be the cause of the neglect of larynx in patients with chronic pharyngitis.Ultra-high power microscope can be used as a new method for screening helicobacter pylori in pharyngeal secretions.The relationship between Helicobacter pylori infection and laryngeal reflux needs to be further studied.

关 键 词：显微镜 幽门螺旋杆菌 咽炎 

分 类 号：R73[医药卫生—肿瘤]