TRPM2在损伤性神经性疼痛过程中参与急性疼痛向慢性疼痛的转化  

TRPM2 participates the transformation of acute pain to chronic pain during injury-induced neuropathic pain

作　　者：何加宁[1] 程涛[1] 黄家骏[1] HE Jia-ning;CHENG Tao;HUANG Jia-jun(Department of pain,Sport Hospital Attached to Chengdu Sport University,Chengdu,Sichuan Province 610041,China)

机构地区：[1]成都体育学院附属体育医院疼痛科,四川成都610041

出　　处：《解剖学研究》2021年第3期213-217,共5页Anatomy Research

摘　　要：目的探究TRPM2在损伤性神经性疼痛中的作用和可能机制。方法建立坐骨神经慢性缩窄性损伤(CCI)大鼠模型,体外培养原代背根神经节(DRG)细胞并完成siRNA的转染,CCI大鼠早期(CCI术后1~4 d)或晚期(CCI术后7~10 d)每天给予阴性对照或si TRPM2处理。RT-PCR检测CCI大鼠DRG和脊髓中TRPM2mRNA的表达,观察大鼠机械痛敏感性和热痛敏感性情况,测定iNOS、NO和ROS水平。结果CCI显著增加DRG和脊髓中TRPM2的表达,CCI术后早期敲除TRPM2可减轻损伤引起的神经性疼痛,而后期敲除则没有效果,CCI大鼠敲除TRPM2可显著降低iNOS的表达、NO以及ROS的水平。结论TRPM2主要参与损伤性神经性疼痛中急性疼痛向慢性疼痛的早期转化,其减轻了早期阶段损伤引起的神经性疼痛,这是一个潜在的神经性疼痛的早期治疗靶点。Objective To explore the role and possible mechanism of TRPM2 in traumatic neuropathic pain.Methods Establish a rat model of chronic constrictive injury of sciatic nerve(CCI).Primary dorsal root ganglion(DRG)cells were cultured in vitro and transfected with siRNA.CCI rats were treated daily with negative control or si TRPM2 either early(1-4 days after CCI)or late(7-10 days after CCI).The expression of TRPM2 in DRG and spinal cord of CCI rats was detected by RT-PCR.The sensitivity of mechanical pain and thermal pain in rats were observed.INOS,NO and ROS levels were measured.Results CCI significantly increased the expression of DRG and TRPM2 in the spinal cord.Early knockout of TRPM2 after CCI could reduce the neuropathic pain caused by injury,while late knockout had no effect.In CCI rats,TRPM2 knockout significantly reduced the level of iNOS expression of NO and ROS.ConclusionTRPM2 is mainly involved in the early transformation of acute pain into chronic pain in traumatic neuropathic pain.TRPM2 alleviates neuropathic pain caused by early stage injury,which is a potential target for early treatment of neuropathic pain.

关 键 词：慢性缩窄性损伤 神经性疼痛 背根神经节 脊髓 坐骨神经 瞬时受体电位M2 

分 类 号：R745[医药卫生—神经病学与精神病学]