毒蕈条盖盔孢伞对小鼠毒性作用研究  

Study on the toxicity of Galerina sulciceps on mice

作　　者：刘泳廷[1] 刘佳[1] 叶建方[1] 李磊[1] 李海蛟[2] 周亚娟[1] LIU Yongting;LIU Jia;YE Jianfang;LI Lei;LI Haijiao;ZHOU Yajuan(Guizhou Provincial Center for Disease Control and Prevention,Guizhou Guiyang 550004,China;National Institute Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention,Beijing 100050,China)

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004 [2]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《中国食品卫生杂志》2021年第3期264-268,共5页Chinese Journal of Food Hygiene

基　　金：贵州省科技厅科学技术项目(黔科合基础[2017]1095)。

摘　　要：目的研究条盖盔孢伞对小鼠的毒性作用。方法采用经鉴定为条盖盔孢伞(Galerina sulciceps)的毒蕈,含α-鹅膏毒肽(α-AMA)为1 690μg/g。小鼠设定464、215、100、46.4 mg/kg BW 4个剂量组,腹腔注射,取心、肝、脾、肺、肾、脑、肠进行病理学诊断检查。结果雌性小鼠半数致死量(LD50)为108 mg/kg BW(可信限:74.1~157 mg/kg BW),小鼠肝脏病理显示随剂量的增加,肝细胞肿胀、坏死,伴有出血和炎细胞浸润的病变逐渐加重。结论条盖盔孢伞主要含有α-鹅膏毒肽,对动物及人可造成肝脏损害。研究条盖盔孢伞的毒性作用机制,对提高中毒者的生存率具有重要意义。Objective To study the toxic effects of Galerina sulciceps on mice. Methods The poisonous mushrooms collected from food poisoning were identified as Galerina sulciceps. The detected α-amanodexin peptide(α-AMA) was 1 690 μg/g. The mice were designed into 4 dose groups of 464, 215, 100, 46.4 mg/kg BW. After intraperitoneal injection, heart, liver, spleen, lung, kidney, brain, and intestine of mice were taken for pathological diagnosis. ResultsIn animal experiments, the median lethal dose(LD50) of female mice was 108 mg/kg BW(confidence limit: 74.1-157 mg/kg BW). The pathology of the liver of mice showed that with the increase of dose, hepatocyte swelling and necrosis, accompanied by bleeding and inflammatory cell infiltration, the pathological changes gradually worsened. Conclusion Galerina sulciceps mainly contains α-AMA, which causes liver damage to humans and animals. Studying the toxic mechanism of Galerina sulciceps has great significance to improve the survival rate of poisoned patients.

关 键 词：条盖盔孢伞 小鼠 毒性作用 

分 类 号：R155[医药卫生—营养与食品卫生学]