cGMP对大鼠酒精偏好的影响  

Effect of cGMP on alcohol preference in rats

作　　者：高青 杨涛 张洪艳[1] 王琦玉 关艳中[1] GAO Qing(Mudanjiang Medical University, Mudanjiang 157011, China)

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2021年第4期8-11,共4页Journal of Mudanjiang Medical University

基　　金：国家自然科学基金面上项目(81871041,81371463)。

摘　　要：目的通过观察大鼠酒精戒断后再饮酒情况,探讨环磷酸鸟苷(cyclic cGMP,cGMP)对大鼠饮酒偏好的影响。方法雄性SD大鼠18只,随机分为对照组(饮水,n=6)和饮酒组(n=12),饮酒组间歇性主动饮20%酒精水溶液28 d,每天记录液体消耗量;建模成功后随机分为末次饮酒组和戒断72 h组,取中脑腹侧被盖区(ventral tegmental area,VTA)脑组织,酶联免疫吸附法(ELISA)检测cGMP含量;雄性SD大鼠30只,间歇性主动饮20%酒精水溶液28 d(第21天时VTA区埋置套管,然后继续间歇性饮酒至28 d),建模成功后酒精戒断72 h,随机分成假手术组(正常做手术但不注药)、cGMP类似物8溴环鸟苷-磷酸(8-Bromoguanosine 3′,5′-cyclic monophosphate sodium salt,8-Br-cGMP)组、人工脑脊液(Artificial cerebrospinal fluid,ACSF)组、鸟苷酸环化酶抑制剂1H-[1,2,4]恶二唑[4,3-a]喹恶啉-1-酮(1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one,ODQ)组、二甲基亚砜(Dimethyl sulphoxide,DMSO)组,每组给予相应的药物,观察给药后大鼠6 h内饮酒偏好情况。结果间歇性主动饮酒28 d,对照组与饮酒组大鼠体重无明显差异(P>0.05);饮酒组大鼠饮酒量及饮酒偏好达到稳定状态,成功建立酒精成瘾模型;酒精戒断72 h时VTA区cGMP含量较戒断前明显增高,有统计学差异(P<0.01);与假手术组和ACSF组比较,8-Br-cGMP组饮酒偏好增高,有统计学差异(P<0.01);与假手术组和DMSO组比较,ODQ组饮酒偏好降低,有统计学差异(P<0.001)。结论大鼠间歇性主动饮酒28 d成功建立酒精成瘾模型;酒精戒断72 h后VTA区cGMP含量增加;cGMP可增加主动饮酒大鼠的饮酒偏好行为。Objective To investigate the effects of cyclic cGMP on alcohol preferences in rats after alcohol withdrawal by observing the re-drinking status of rats.Methods 18 male SD rats were randomly divided into control group(water,n=6)and drinking group(n=12),alcohol drinking way of intermittent active 20%alcohol aqueous solution 28 days,daily record liquid consumption.After the success of the model,all rats were randomly divided into group and alcohol intake in withdrawal at the end of the group of 72 hours,the midbrain ventral tegmental area(ventral tegmental area,the VTA)brain tissue were collected,and the method of enzyme-linked immunosorbent(ELISA)was used to detect the contents of cGMP.30 adult male SD rats,intermittent active drink 20%alcohol aqueous solution for 28 days(21 days VTA area was embedded casing,then continue to intermittent drinking).After 72 hours since the success of the modeling of alcohol withdrawal,the rats were randomly divided into control group(normal operation,but not note medicine),8-Br-cGMP group,ACSF group,ODQ group and DMSO group,tracking dosing within 6 hours of rats after drinking preferences.Results There was no difference in body weight between the control group and the experimental group(P>0.5);The alcohol consumption and drinking preference of rats in the drinking group reached a stable state,and the alcohol addiction model was successfully established.The content of cGMP in VTA increased significantly after 72 h of alcohol abstinence(P<0.01);Compared with the sham operation group and the ACSF group,the drinking preference of the 8-Br-cGMP group was increased,and the difference was statistically significant(P<0.01);Compared with the sham operation group and the DMSO group,the ODQ group had a decreased drinking preference,and the difference was statistically significant(P<0.001).Conclusion We successfully established the model of alcohol addiction in rats after intermittent and active drinking for 28 days.The content of cGMP increased in VTA area 72 hours after alcohol withdrawal,and

关 键 词：CGMP 酒精戒断 饮酒偏好 

分 类 号：R749.62[医药卫生—神经病学与精神病学]