不同解冻程序对荣昌猪冷冻精液解冻效果的影响  

Effect of Different Thawing Procedures on the Thawing Rongchang Pigs Frozen Semen

作　　者：甘麦邻 刘麟 赵雪[1,2] 郑婷 郭宗义 沈林園[1,2] 潘红梅[3] 张亮 王金勇[3] 朱砺[1,2] GAN Mailin;LIU Lin;ZHAO Xue;ZHENG Ting;GUO Zongyi;SHEN Linyuan;PAN Hongmei;ZHANG Liang;WANG Jinyong;ZHU Li(College of Animal Science and Technology,Sichuan Agricultural University,Sichuan Chengdu 611130,China;Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Sichuan Chengdu 611130,China;Chongqing Academy of Animal Science,Chongqing 402460,China)

机构地区：[1]四川农业大学动物科技学院,四川成都611130 [2]四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室,四川成都611130 [3]重庆市畜牧科学院,重庆402460

出　　处：《中国畜牧杂志》2021年第7期140-146,共7页Chinese Journal of Animal Science

基　　金：国家重点研发计划项目(2018YFD0501004);国家现代农业产业技术体系四川生猪创新团队项目(SCSZTD-3-008);四川省科技支撑计划项目(2016NYZ0050);重庆市社会事业与民生保障科技创新专项项目(cstc2017-zdyf0247)。

摘　　要：为优化猪冷冻精液解冻程序,本研究使用3种解冻液(M、J和C)对荣昌猪冷冻精液进行解冻,检测冷冻精液在不同解冻温度(32、37、42、47、52℃)和不同解冻时间(10 s或20 s)解冻后的精子活率、精子活力、快速前进运动精子数和慢速前进运动精子数,并使用因子分析法评估不同解冻液最佳解冻程序。结果显示:解冻液M在保存指标和持续指标方面较优;解冻液J在即时指标和持续指标方面较优;解冻液C在持续指标方面较优。综合分析表明,解冻液M和解冻液J的最佳解冻程序为42℃水浴解冻20 s,解冻液C的最佳解冻程序为32℃水浴解冻20 s。In order to optimize the thawing procedure of pig frozen semen,this study used three thawing fluids(M,J and C)to defrost the frozen semen of Rongchang pigs,and detected the frozen semen at different thawing temperatures(32℃,37℃,42℃,47℃ and 52℃)and different thawing time(10 s or 20 s)after thawing,sperm motility,sperm motility,number of fastmoving sperm and slow-moving sperm,and using factor analysis to evaluate the best thawing of different thawing solutions program.The results show that thawing solution M is better in terms of preservation index and continuous index;thawing solution J is better in terms of immediate index and continuous index;thawing solution C is better in terms of continuous index.Comprehensive analysis found that the best thawing program for thawing solution M and thawing solution J was 42℃ water bath thawing for 20 s,and the best thawing program for thawing solution C was 32℃ water bath thawing for 20 s.

关 键 词：荣昌猪 冷冻精液 解冻液 解冻程序 

分 类 号：S828.3[农业科学—畜牧学]