不同分子质量丝素蛋白的分离与表征  

Separation and characterization of silk fibroin with different molecular weight

作　　者：丁梦瑶 戴梦男 李蒙 刘苹 徐晶晶[1] 王建南[1,2] DING Mengyao;DAI Mengnan;LI Meng;LIU Ping;XU Jingjing;WANG Jiannan(College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China;National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China)

机构地区：[1]苏州大学纺织与服装工程学院,江苏苏州215123 [2]苏州大学现代丝绸国家工程实验室,江苏苏州215123

出　　处：《纺织学报》2021年第7期46-53,共8页Journal of Textile Research

基　　金：国家自然科学基金项目(51873141,51473108);江苏省自然科学基金项目(BK20181192);江苏省高等学校自然科学研究重大项目(18KJA540001)。

摘　　要：为获得不同分子质量且分布相对集中的丝素蛋白,采用葡聚糖凝胶层析技术对再生丝素蛋白溶液进行梯度纯化、分离。通过使用合适的葡聚糖凝胶并控制层析流速,有效分离出不同分子质量的丝素蛋白,并进一步对获得的4组不同分子质量的丝素蛋白的黏度、电负性、氨基酸组成和分子构象进行分析。结果表明:丝素蛋白溶液的黏度和等电点随其分子质量的减小而减小,其电负性的提高主要源自于丝素重链被切断,且富含非重复区域的较小分子质量的肽链;4组不同分子质量的丝素蛋白均可自组装形成一定的β-折叠结构,分子质量越高的丝素蛋白越易形成稳定的分子构象。The sephadex gel chromatography was used for gradient purification and separation to obtain relatively centralized silk fibroin with different molecular weight distributions from regenerated silk fibroin.The silk fibroin with different molecular weights was separated effectively by using the appropriate sephadex gel and controlling the chromatographic flow rate.The viscosity,electronegativity,amino acid composition and molecular conformation of the preliminarily separated four silk fibroins were further analyzed.Results showed that the viscosity and isoelectric point of the silk fibroin solution decreased with the decrease of molecular weight.The increased electronegativity is mainly due to the silk fibroin heavy chain which were cut off into smaller peptide chains richly exists in non-repetitive regions.Each of the four silk fibroins in the study could formβ-sheet structure by self-assemble,and the higher the molecular weight,the easier it was to form a stable molecular conformation.

关 键 词：丝素蛋白 凝胶层析 分子质量 两性性质 分子构象 

分 类 号：TS141.8[轻工技术与工程—纺织材料与纺织品设计] Q816[轻工技术与工程—纺织科学与工程]