Revealing HOC1 burst from endoplasmic reticulum in cisplatin-treated cells via a ratiometric fluorescent probe  

作　　者：Shan Wang Beitong Zhu Bingya Wang Xinhua Cao Lei Zhu Ji-Ting Hou Lintao Zeng 

机构地区：[1]Institute of Biomedical Materials Industry Technology,Hubei Engineering University,Xiaogan 432000,China [2]College of Light Industry and Food Engineering,Cuangxi University,Nanning 530004,China [3]Key Laboratory of Emergency and Trauma,Ministry of Education,Key Laboratory of Hainan Trauma and Disaster Rescue,College of Emergency and Trauma,Hainan Medical University,Haikou 571199,China [4]College of Chemistry and Chemical Engineering,Xinyang Normal University,Xinyang 464000,China

出　　处：《Chinese Chemical Letters》2021年第5期1795-1798,共4页中国化学快报（英文版）

基　　金：This work was supported by the Hubei Provincial Department of Education Science and Technology Research Projects(No.Q20182704).

摘　　要：The reactive oxygen species(ROS) are tightly associated with endoplasmic reticulum(ER) stress.Thus,the deep and visual insight of aberrant ROS fluctuations in the ER can help us better investigate the ER stress-associated pathology.In this work,a fluorescent probe ERC for HOCI detection in the ER based on phenothiazine-derived coumarin platform was developed.In the presence of HOCI,ERC exhibited an emission change from 609 nm to 503 nm within seconds.It also showed high sensitivity(0.44 μmol/L)and superb photostability.Significantly,ERC displayed low cytotoxicity,good cell membrane permeability,and appreciable ER-targetability.Ultimately,the probe was successfully utilized to image exogenous and endogenous HOCl in living cells and reveal the HOCI burst in cisplatin-treated cancer cells.

关 键 词：Fluorescent probes RATIOMETRIC HOCL Endoplasmic reticulum CISPLATIN 

分 类 号：O657.3[理学—分析化学] R730.53[理学—化学]