Molecular recognition triggered aptazyme cascade for ultrasensitive detection of exosomes in clinical serum samples  

作　　者：Kemei Jiang Yanan Wu Juan Chen Mingqing Shi Hong-Min Meng Zhaohui Li 

机构地区：[1]College of Chemistry,Green Catalysis Center,Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalyrical Applications,Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic,Zhengzhou University,Zhengzhou 450001,China

出　　处：《Chinese Chemical Letters》2021年第5期1827-1830,共4页中国化学快报（英文版）

基　　金：This work was supported by National Natural Science Founda-tion of China(Nos.21605038 and 21974125);China Postdoctoral Science Foundation(No.2019T120623).

摘　　要：Exosomes have attracted widespread interest due to their inherent advantages in tumor diagnosis and treatment monitoring.However,it is still a big challenge for highly sensitive and specific detection of exosome in real complexed samples.Herein,a molecular recognition triggered aptazyme cascade strategy was developed for ultrasensitive detection of cancer exosomes in clinical serum samples.In this design,one target exosome could capture a large quantity of aptazymes for the first-step signal amplification.And then the captured aptazyme was activated and recycled to release the fluorophore-labelled substrate strand for a cascaded signal amplification.Notably,the activation of aptazyme only occurs whenithas bound with target exosome,ensuring a low background.The experimental results show that the limit of detection(LOD)and the limit of quantification(LOQ)are 3.5×10^(3) particles/μL and 1.7×10^(4) particles/μL,respectively,which is comparable to the results of most existed fluorescence-based exosome probes.Moreover,this assay possesses high specificity to distinguish exosomes derived from other cell lines.Furthermore,this fluorescence probe was utilized in cancer patient and healthy serum samples successfully,suggesting its great potential for clinical diagnosis and biological studies.

关 键 词：Aptazyme EXOSOMES Fluorescence probe Signal amplification Clinical serum samples 

分 类 号：O657.3[理学—分析化学] R446.1[理学—化学]