FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring  

作　　者：Yuzhen Ouyang Yanping Liu Zhiming MWang Zongwen Liu Minghua Wu 

机构地区：[1]Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya Medical School,Central South University,Changsha 410013,Hunan,People’s Republic of China [2]School of Physics and Electronics,Hunan Key Laboratory for Super-Microstructure and Ultrafast Process,Central South University,932 South Lushan Road,Changsha 410083,Hunan,People’s Republic of China [3]The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health,The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education,Cancer Research Institute,Central South University,Changsha 410008,Hunan,People’s Republic of China [4]Shenzhen Research Institute of Central South University,A510a,Virtual University Building,Nanshan District,Southern District,High-tech Industrial Park,Yuehai Street,Shenzhen,People’s Republic of China [5]State Key Laboratory of High-Performance Complex Manufacturing,Central South University,932 South Lushan Road,Changsha 410083,Hunan,People’s Republic of China [6]Institute of Fundamental and Frontier Sciences,University of Electronic Science and Technology of China,Chengdu 610054,Sichuan,People’s Republic of China [7]School of Chemical and Biomolecular Engineering,The University of Sydney,Sydney,NSW 2006,Australia

出　　处：《Nano-Micro Letters》2021年第9期48-74,共27页纳微快报（英文版）

基　　金：This work was partially supported by the National Natural Science Foundation of China(Grant No.61775241);the Hunan Science Fund for Distinguished Young Scholar(2020JJ2059);Youth Innovation Team(Grant No.2019012)of CSU,Hunan province key research and development project(Grant No.2019GK2233,Grant 2020SK2053);Hunan Province Graduate Research and Innovation Project(Grant No.CX20190177);the Science and Technology Innovation Basic Research Project of Shenzhen(Grant No.JCYJ20180307151237242);Also,YPL acknowledges the support by the Project of State Key Laboratory of High-Performance Complex Manufacturing,Central South University(Grant No.ZZYJKT2020-12).Besides,we acknowledge the art work from Servier Medical Art.Y.Z.O and Y.P.L contributed equally to this work.

摘　　要：Fluorescence lifetime imaging microscopy(FLIM)has been rapidly developed over the past 30 years and widely applied in biomedical engineering.Recent progress in fluorophore-dyed probe design has widened the application prospects of fluorescence.Because fluorescence lifetime is sensitive to microenvironments and molecule alterations,FLIM is promising for the detection of pathological conditions.Current cancer-related FLIM applications can be divided into three main categories:(i)FLIM with autofluorescence molecules in or out of a cell,especially with reduced form of nicotinamide adenine dinucleotide,and flavin adenine dinucleotide for cellular metabolism research;(ii)FLIM with Förster resonance energy transfer for monitoring protein interactions;and(iii)FLIM with fluorophore-dyed probes for specific aberration detection.Advancements in nanomaterial production and efficient calculation systems,as well as novel cancer biomarker discoveries,have promoted FLIM optimization,offering more opportunities for medical research and applications to cancer diagnosis and treatment monitoring.This review summarizes cutting-edge researches from 2015 to 2020 on cancer-related FLIM applications and the potential of FLIM for future cancer diagnosis methods and anti-cancer therapy development.We also highlight current challenges and provide perspectives for further investigation.

关 键 词：Fluorescence lifetime imaging microscopy Förster resonance energy transfer Reduced form of nicotinamide adenine dinucleotide Biosensors CANCER 

分 类 号：TH77[机械工程—仪器科学与技术]