阿帕替尼对胃癌细胞增殖、凋亡及VEGFR通路的影响研究  

Effect research of apatinib on the proliferation,apoptosis and VEGFR pathway of gastric cancer cells

作　　者：宋锦添 陈奕贵[1] 郑亮[1] 蔡雄超[1] 王益[2] SONG Jintian;CHEN Yigui;ZHENG Liang;CAI Xiongchao;WANG Yi(Department of Abdominal Oncology,the Affiliated Cancer Hospital of Fujian Medical University&Fujian Cancer Hospital,Fuzhou 350014,Fujian province,China;Department of Abdominal Tumor Surgery,the Affiliated Cancer Hospital of Fujian Medical University&Fujian Cancer Hospital,Fuzhou 350014,Fujian province,China)

机构地区：[1]福建医科大学附属肿瘤医院&福建省肿瘤医院腹部肿瘤内科,福州市350014 [2]福建医科大学附属肿瘤医院&福建省肿瘤医院腹部肿瘤外科,福州市350014

出　　处：《内科》2021年第3期303-307,共5页Internal Medicine

基　　金：福建省卫生健康青年科研课题(2019-1-10)。

摘　　要：目的探讨阿帕替尼对胃癌细胞增殖、凋亡及VEGFR通路的影响。方法选取胃癌细胞系HGC-27和NCI-N87细胞,通过CCK-8细胞增殖试验和克隆形成实验探讨不同浓度阿帕替尼对HGC-27和NCI-N87细胞增殖的影响;采用流式细胞术检测不同浓度阿帕替尼对HGC-27和NCI-N87细胞凋亡的影响;采用Western blot法检测不同浓度阿帕替尼对HGC-27和NCI-N87细胞凋亡相关蛋白(Bcl-2、Bax、Caspase 3、Cleaved-Caspase 3)以及血管内皮生长因子受体2(VEGFR2)表达量的影响。结果CCK-8细胞增殖试验和克隆形成实验结果显示,随着阿帕替尼浓度的增加,NCI-N87、HGC-27细胞存活率、细胞克隆数逐渐下降。流式细胞术检测结果显示,随着阿帕替尼浓度的增加,NCI-N87及HGC-27细胞凋亡率显著增加。Western blotting结果显示,随着阿帕替尼浓度的增加,NCI-N87及HGC-27细胞的Bcl-2表达水平降低,Bax、Cleaved-Caspase 3的表达水平升高;随着阿帕替尼浓度升高,NCI-N87及HGC-27细胞的VEGFR2表达水平下降。结论阿帕替尼具有抑制胃癌细胞增殖、促进其凋亡的作用,可能与其可调节凋亡相关蛋白和VEGFR通路相关蛋白的表达水平有关。Objective To explore the effects of apatinib on the proliferation,apoptosis and the VEGFR pathway of gastric cancer cells.Methods HGC-27 and NCI-N87 cells belonged to gastric cancer cell lines were selected,and the effects of different concentrations of apatinib on the proliferation of HGC-27 and NCI-N87 cells were investigated by CCK-8 cell proliferation test and clone formation test.Flow cytometry was used to detect the effect of apatinib in different concentrations on the apoptosis of HGC-27 and NCI-N87 cells.Western blot was used to detect the effects of different concentrations of apatinib on the apoptosis-related proteins(Bcl-2,Bax,Caspase 3,Cleaved-Caspase 3)and vascular endothelial growth factor receptor 2(VEGFR2)expression of HGC-27 and NCI-N87 cells.Results The results of CCK-8 cell proliferation test and clone formation test showed that,with the increase of the apatinib concentrations,the survival rate of NCI-N87 and HGC-27 cells and the number of cell clones gradually decreased.The detective results of flow cytometry expressed that along with the increase of the concentrations of apatinib,the apoptosis rate of NCI-N87 and HGC-27 cells increased significantly.Western blot results revealed that as the concentrations of apatinib increased,the expression level of Bcl-2 decreased,whereas the expression levels of Bax and Cleaved-Caspase 3 increased,and the expression level of VEGFR2 decreased in both NCI-N87 and HGC-27 cells.Conclusion Apatinib can inhibit the proliferation of gastric cancer cells and promote apoptosis,which may be related to its ability to regulate the expression levels of apoptosis-related proteins and VEGFR pathway-related proteins.

关 键 词：胃癌 VEGFR通路 增殖 凋亡 阿帕替尼 

分 类 号：R735.2[医药卫生—肿瘤]