吡非尼酮对人胆管癌HuCCT1细胞增殖、迁移及侵袭的影响  

The effect of pirfenidone on proliferation,migration and invasion of cholangiocarcinoma HuCCT1 cells

作　　者：李丽萍 干小红[1] 周永杰[2] 周后凤[1] LI Liping;GAN Xiaohong;ZHOU Yongjie;ZHOU Houfeng(Department of Pharmacy, Chengdu Fifth People's Hospital, Chengdu 611130, China;Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHC, West China Hospital, Sichuan University, Chengdu 610041, China)

机构地区：[1]成都市第五人民医院药剂科,四川成都611130 [2]四川大学华西医院卫生部移植工程与移植免疫重点实验室病理研究室,四川成都610041

出　　处：《西部医学》2021年第7期953-957,共5页Medical Journal of West China

基　　金：国家自然科学基金(81800449)。

摘　　要：目的探究吡非尼酮(PFD)对人胆管癌HuCCT1细胞增殖、迁移及侵袭能力的影响。方法CCK-8法检测不同浓度PFD(0、0.25、0.5、0.75、1 g/L)对HuCCT1细胞增殖的抑制情况;平板克隆形成实验检测PFD对HuCCT1细胞克隆形成能力的影响;划痕修复实验和Transwell实验检测PFD对HuCCT1细胞迁移及侵袭能力的影响;Western blot检测PFD对HuCCT1细胞上皮间质转化(EMT)标志分子N-cadherin、E-cadherin及Vimentin蛋白表达水平的影响。结果与对照组相比,PFD显著抑制HuCCT1细胞的增殖及克隆形成能力(P<0.05);划痕修复实验及Transwell实验结果显示PFD抑制HuCTT1细胞的迁移及侵袭能力(P<0.05);Western blot实验结果显示PFD显著上调HuCCT1细胞E-cadherin蛋白表达水平,同时下调N-cadherin、Vimentin蛋白表达水平(P<0.05)。结论PFD抑制胆管癌HuCCT1细胞的增殖、迁移、侵袭及EMT进程。Objective To investigate the effect of pirfenidone(PFD)on proliferation,migration and invasion of cholangiocarcinoma HuCCT1 cells.Methods The proliferation of HuCCT1 cells treated with various concentration(0,0.25 g/L,0.5 g/L,0.75 g/L,1 g/L)was assessed by CCK-8.The effect of PFD on clone ability of HuCCT1 was analyzed by clonal assay.The motility of PFD treatment on HuCCT1 cells was determined by wound healing assay and transwell assay.The expression levels of EMT markers were detected by western blot.Results PFD significantly decreased the proliferation of HuCCT1 cells in a concentration-dependent manner compared with control group(P<0.05).The colony formation was reduced in HuCCT1 cells treated with PFD(P<0.05).The migration and invasion ability of HuCCT1 cells treated with PFD were also inhibited(P<0.05).The results of western blot showed that the expression of E-cadherin was up-regulated and the expression of N-cadherin and Vimentin down-regulated in PFD-treated cells compared with control group(P<0.05).Conclusion PFD inhibits the proliferation,migration,invasion and EMT process of cholangiocarcinoma HuCCT1 cells.

关 键 词：吡非尼酮 胆管癌 细胞增殖 细胞迁移 细胞侵袭 上皮间质转化 

分 类 号：R73-37[医药卫生—肿瘤] R735.8[医药卫生—临床医学]