Nb_(0.12)-TiO_(2)对脑胶质瘤的光热治疗及乏氧微环境的影响  

Effects of Nb-TiO on the photothermal therapy and hypoxic microenvironment of glioma

作　　者：孙娜[1] 耿鹏 赵凌舟[1] 龚佳丽 邢岩[1] 陈志钢[2] 赵晋华[1] SUN Na;GENG Peng;ZHAO Lingzhou;GONG Jiali;XING Yan;CHEN Zhigang;ZHAO Jinhua(Department of Nuclear Medicine,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China;College of Materials Science and Engineering,Donghua University,Shanghai 201620,China)

机构地区：[1]上海交通大学附属第一人民医院核医学科,上海200080 [2]东华大学材料科学与工程学院,上海201620

出　　处：《肿瘤影像学》2021年第3期149-154,共6页Oncoradiology

基　　金：国家自然科学基金(81801727)。

摘　　要：目的:探讨新型光敏纳米材料铌-二氧化钛(Nb_(0.12)-TiO_(2))在近红外光(near-infrared light,NIR)激发下对脑胶质瘤的光热治疗效果和对低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)的影响。方法:使用细胞计数试剂盒-8(cell counting kit-8,CCK-8)法评估Nb_(0.12)-TiO_(2)在无NIR激发下对人脑胶质瘤细胞(U87细胞)活性的影响;活死细胞染色评估Nb_(0.12)-TiO_(2)对U87细胞的光热治疗效果;实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)评估U87细胞经Nb_(0.12)-TiO_(2)光热治疗后HIF-1α的基因表达水平。结果:CCK-8法结果表明,Nb_(0.12)-TiO_(2)与U87细胞共培养24 h后,细胞活性>90%。活死细胞染色结果显示,在磷酸盐缓冲生理盐水(phosphate-buffered saline,PBS)组、Nb_(0.12)-TiO_(2)组和PBS+NIR组未出现明显的细胞凋亡,而Nb_(0.12)-TiO_(2)+NIR组出现大量细胞死亡。RTFQ-PCR实验发现,U87细胞经Nb_(0.12)-TiO_(2)+NIR治疗后,HIF-1α的基因表达大幅下调,与PBS组、Nb_(0.12)-TiO_(2)组和PBS+NIR组差异有统计学意义(P<0.001)。结论:Nb_(0.12)-TiO_(2)具有高度的生物相容性,在NIR激发下可显著杀伤U87细胞,且可同时下调HIF-1α的表达,有望用于脑胶质瘤的光热治疗。Objective:To evaluate the photothermal therapy effects of a new types of photosensitive nanomaterial,niobiumdoped titanium dioxide(Nb_(0.12)-TiO_(2)),on gliomas under the excitation of near-infrared light(NIR)and the effects of Nb_(0.12)-TiO_(2)on hypoxia inducible factor-1α(HIF-1α).Methods:Cell counting kit-8(CCK-8)assays was used to evaluate the effects of Nb_(0.12)-TiO_(2)on the viability of human glioma cells(U87 cells)without NIR stimulation.The photothermal effect of Nb_(0.12)-TiO_(2)on U87 cells was evaluated by live and dead cell double staining.The gene level of HIF-1αin U87 cells after photothermal treatment with Nb_(0.12)-TiO_(2)was evaluated by real-time fluorescence quantitative polymerase chain reaction(RTFQ-PCR).Results:CCK-8 assay showed that after cocultured with Nb_(0.12)-TiO_(2)for 24 h,the viability of U87 cells was>90%.The results of live and dead cell double staining showed that a large number of cell death occurred in Nb_(0.12)-TiO_(2)+NIR group,and there was no obvious apoptosis in phosphate-buffered saline(PBS)group,Nb_(0.12)-TiO_(2)group and PBS+NIR group.RTFQ-PCR showed that the expression of HIF-1αwas significantly downregulated in U87 cells after treated with Nb_(0.12)-TiO_(2)+NIR,which was significantly different from that in PBS group,Nb_(0.12)-TiO_(2)group,and PBS+NIR group(P<0.001).Conclusion:Nb_(0.12)-TiO_(2),with high biocompatibility,can significantly kill U87 cells and downregulate the expression of HIF-1αunder the excitation of NIR,which is expected to be used in the photothermal treatment of glioma in future.

关 键 词：脑胶质瘤 铌-二氧化钛 光热治疗 低氧诱导因子-1Α 

分 类 号：R739.41[医药卫生—肿瘤]