蛹虫草HPLC特征图谱的建立及其方法学研究  

Establishment of HPLC Characteristic Chromatogram of Cordyceps Militaris and Its Methodology

作　　者：史文婷 徐崇范[2] 门英[2] 丁桂兰[2] 魏春雁[3] SHI Wenting;XU Chongfan;MEN Ying;DING Guilan;WEI Chunyan(Clinical Medicine College,Changchun University of Chinese Medicine,Changchun 130117;Jilin Provincial Institute for Drug Control,Changchun 130033;Jilin Academy of Agricultural Sciences/Risk Assessment Lab of Agriproducts Quality and Safety(Changchun),Ministry of Agriculture and Rural Affairs of the P.R.C.,Changchun 130033,China)

机构地区：[1]长春中医药大学临床医学院,长春130117 [2]吉林省药品检验所,长春130033 [3]吉林省农业科学院/农业农村部农产品质量安全风险评估实验室(长春),长春130033

出　　处：《东北农业科学》2021年第3期134-138,共5页Journal of Northeast Agricultural Sciences

基　　金：吉林省科技厅项目(20150201007YY);农业农村部农产品质量安全风险评估重大专项(GJFP2017006)。

摘　　要：为准确有效鉴别蛹虫草,采用Luna C18(2)色谱柱(250 mm×4.6 mm,5μm),以甲醇-pH 6.5磷酸盐缓冲液为流动相,流速0.5 mL/min,柱温30℃,梯度洗脱,260 nm波长检测,建立了蛹虫草HPLC特征图谱。以鸟苷、尿苷、胞苷、腺苷、腺嘌呤、虫草素6种标准品做对照,指认了13批样品中的共有特征色谱峰8个,并指认其中的鸟苷峰为参照峰。该方法的精密度、重现性和稳定性均符合要求。该方法可用于蛹虫草的定性鉴别和质量评价。In order to accurately and effectively identify Cordyceps militaris, its HPLC characteristic chromatogram was established with Luna C18(2)(250 mm×4.6 mm, 5 μm) column in a gradient elution of methanol-phosphate buffer(pH 6.5), at the flow rate of 0.5 mL/min and 30 ℃ column temperature. Guanosine, uridine, cytidine, adenosine,adenine and cordycepin were used as reference standard. Total 8 characteristic peaks in 13 batches of samples were identified and the guanosine peak was identified as the reference peak. The precision, repeatability and stability of this method were all met with the requirements. This method could be used for qualitative identification and quality evaluation of C. militaris.

关 键 词：蛹虫草 HPLC特征图谱 方法学 

分 类 号：R917[医药卫生—药物分析学]