人参水溶性膳食纤维对大鼠糖脂代谢、氧化应激和肠道健康的影响  

Effect of Ginseng Water-Soluble Dietary Fiber on Glucolipid Metabolism,Oxidative Stress and Intestinal Health in Rats

作　　者：华梅 樊美玲[2] 李志满 董丽娜 李珊珊[1] 孙印石[1] HUA Mei;FAN Meiling;LI Zhiman;DONG Lina;LI Shanshan;SUN Yinshi(Institute of Special Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun 130112,China;Jilin Academy of Chinese Medicine Sciences,Changchun 130021,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112 [2]吉林省中医药科学院,吉林长春130021

出　　处：《食品科学》2021年第13期127-135,共9页Food Science

基　　金：中央级公益性科研院所基本科研业务费专项(1610342020005;1610342019008);吉林省科技发展计划医药健康专项(20191102052YY)。

摘　　要：目的:研究人参水溶性膳食纤维对健康大鼠糖脂代谢、氧化应激和肠道健康的影响。方法:向SD大鼠连续灌胃人参水溶性膳食纤维15 d,对照组给予等体积生理盐水,分析大鼠生长情况、代谢水平和肠道菌群结构变化。结果:人参水溶性膳食纤维能够极显著提高大鼠采食量(P<0.01),但对体质量增长率、饲料利用率及脏器指数无显著影响(P>0.05)。低剂量人参水溶性膳食纤维能够显著提高大鼠空腹胰岛素水平(P<0.05),降低血清总胆固醇浓度(P<0.05);高剂量人参水溶性膳食纤维能够显著降低空腹血糖浓度(P<0.05),极显著降低血清总甘油三酯浓度(P<0.01)。人参膳食纤维极显著降低血清丙二醛浓度并提高总抗氧化能力(P<0.01)。低剂量人参水溶性膳食纤维显著降低大鼠炎症因子肿瘤坏死因子α(P<0.01)、白细胞介素1β(P<0.05)和白细胞介素4(P<0.01)水平,并显著提高粪便水分质量分数(P<0.05)。人参水溶性膳食纤维主要增加了厚壁菌门(Firmicutes)和乳杆菌属(Lactobacillus)丰度,使肠道菌群结构向益生菌和纤维素降解菌增殖的方向改变,同时对相关代谢通路功能基因的表达产生影响。结论:人参水溶性膳食纤维能够通过调节糖脂代谢水平、氧化应激状态和肠道菌群结构对健康大鼠产生积极影响。Purpose:To investigate the effect of ginseng water-soluble dietary fiber(GSDF)on glucolipid metabolism and intestinal health in rats.Methods:SD rats were gavaged with GSDF or an equal volume of normal saline as a control for 15 days,and they were evaluated for growth performance,metabolism level and intestinal flora structure.Results:GSDF could significantly increase the feed intake of rats(P<0.01),but had no significant effect on percent body mass gain,feed utilization rate or visceral organ indexes(P>0.05).Low-dose GSDF significantly increased fasting insulin level(P<0.05),and decreased total cholesterol level(P<0.05).High-dose GSDF significantly reduced fasting blood glucose(P<0.05)and total triglyceride level(P<0.01).GSDF significantly reduced serum malondialdehyde content(P<0.01),and improved total antioxidant capacity(P<0.01).Low-dose GSDF significantly reduced the inflammatory factor levels including tumor necrosis factorα(P<0.01),interleukin(IL)1β(P<0.05)and IL-4(P<0.01),and increased fecal water content(P<0.05).Moreover,GSDF increased the abundance of intestinal Firmicutes and Lactobacillus,changing the structure of intestinal flora in the direction of enriching probiotics and cellulose-degrading bacteria,and affected the functional gene expression of related metabolic pathways.Conclusion:GSDF exerts a positive effect on rats by regulating growth metabolism,oxidative stress status and intestinal flora structure.

关 键 词：人参水溶性膳食纤维 生长性能 糖脂代谢 氧化应激 肠道健康 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]