Influence of herb-partitioned moxibustion at Qihai (CV6) and bilateral Tianshu (ST25) and Shangjuxu (ST37) acupoints on toll-like receptors 4 signaling pathways in patients with ulcerative colitis  

作　　者：QI Qin Hyoyoung Im LI Kunshan GU Muen WU Huangan YANG Ling HUANG Yan ZHAO Jimeng CUI Yunhua LIU Huirong WU Luyi 

机构地区：[1]Key Laboratory of Acupuncture and Immunological Effects,Shanghai University of Traditional Chinese Medicine,Shanghai 200030,China

出　　处：《Journal of Traditional Chinese Medicine》2021年第3期478-484,共7页中医杂志（英文版）

基　　金：Supported by grants from the National Natural Science Foundation of China (No.81674074, 81774405, 81704161);three-year Action Plan Project of Shanghai Traditional Chinese Medicine Development [No.ZY(2018-2020)-CCCX-2004-01];project of Shanghai Municipal Health Commission (No.201840096);shanghai Municipal population and Family planning Commission "Excellent Youth Talent Training Program"(No.2018YQ11);Special Clinical Research Project in the Health Industry of Shanghai Municipal Health Commission (No.20204Y0245)。

摘　　要：OBJECTIVE: To evaluate the efficacy of herb-partitioned moxibustion(HPM) at Qihai(CV6), Tianshu(ST25) and Shangjuxu(ST37) acupoints in relieving symptoms and the immune regulation of HPM on the toll-like receptors 4(TLR4) signaling pathway in ulcerative colitis(UC) patients.METHODS: A randomized, single-blind study was conducted 63 patients to receive HPM or sham HPM treatment. The efficacy outcomes included scores of the Mayo, Baron, inflammatory bowel disease questionnaire(IBDQ), self-rating depression scale(SDS), self-rating anxiety scale(SAS). HE staining was used to observe the histopathological changes of the colon. The expression of inflammatory cytokines and TLR4 signaling pathway related molecules were determined by enzyme-linked immunosorbent assay and immunohistochemistry.RESULTS: Baron, SDS, SAS scores were significantly decreased in moxibustion group(P < 0.05), IBDQ score was significantly greater in the moxibustion group than in the sham moxibustion group(P < 0.05). Histopathology of mucosal biopsies showed that both two groups improved in mucosa after treatment. The expression levels of tumor necrosis factor-α, interleukin-2, interleukin-12, interferon-γ, and TLR4, lipopolysaccharide, myeloid differentiation factor 88, interleukin receptor associated kinase, tumor necrosis factor receptor associated factor 6 and nuclear factor kappa-B p65 were significantly lower in the moxibustion group than in the sham moxibustion group(P < 0.05).CONCLUSION: This study showed that HPM at Qihai(CV6), Tianshu(ST25) and Shangjuxu(ST37) acupoints is effective to relieve symptoms, anxiety, depression and improving life quality in UC patients,which may be related to the immune regulation of HPM on TLR4 signaling pathway.

关 键 词：colitis ULCERATIVE MOXIBUSTION toll-like receptors 4 Mayo score Baron score 

分 类 号：R246.1[医药卫生—针灸推拿学]