青贮专用复合酶制剂调控苜蓿发酵品质及营养成分  

Silage-specific compound enzyme regulate alfalfa silage quality and nutrient components

作　　者：李玉玉 牟怡晓 张硕 马聪慧 雷晓青 朱鸿福 张欢 张桂杰[1,2] LI Yuyu;MU Yixiao;ZHANG Shuo;MA Conghui;LEI Xiaoqing;ZHU Hongfu;ZHANG Huan;ZHANG Guijie(Department of Animal Science,Ningxia University,Yinchuan 750021,Ningxia,China;Ningxia Grassland and Animal Husbandry Engineering Technology Research Center,Yinchuan 750021,Ningxia,China;Winning of Tianjin Biotechnology Industry Company,Tianjin 300354,China)

机构地区：[1]宁夏大学农学院动物科学系,宁夏银川750021 [2]宁夏草牧业工程技术研究中心,宁夏银川750021 [3]天津云力之星生物科技有限公司,天津300354

出　　处：《草业科学》2021年第7期1402-1410,共9页Pratacultural Science

基　　金：国家自然科学基金(31660694);宁夏高等学校一流学科建设(草学学科)资助(NXYLXK2017A01)。

摘　　要：为探究青贮专用复合纤维素酶对紫花苜蓿(Medicago sativa)青贮品质及营养成分的影响,按单因素完全随机设计4个处理组,每个处理6个重复。试验所用青贮专用复合酶制剂中纤维素酶活力≥60000 U·g^(−1),木聚糖酶活力≥2500 U·g^(−1),淀粉酶活力≥300000 U·g^(−1),β-葡聚糖酶活力≥300000 U·g^(−1)。4个处理组的青贮专用复合纤维素酶添加量分别为0(T1组)、2.5‰(T2组)、5.0‰(T3组)和7.5‰(T4组)。结果表明:青贮60 d后,青贮专用复合纤维素酶显著提高T4组粗蛋白含量(P<0.05);T3组和T4组的酸性洗涤纤维极显著低于T1组(P<0.01),其相对饲喂价值极显著高于T1组(P<0.01);青贮专用复合纤维素酶极显著降低了苜蓿的青贮pH(P<0.01);T2组和T3组乳酸含量及乳酸/乙酸值显著高于T1组(P<0.05);T2组和T3组有氧暴露稳定时间显著低于T1组和T4组(P<0.05)。综上,在本研究条件下,添加青贮专用复合纤维素酶可改善苜蓿青贮饲料发酵品质,提高苜蓿青贮饲料的营养价值,适宜添加量为5.0‰。This study aimed at evaluating the effects of silage-specific mixed cellulase on alfalfa silage quality and nutrient components.We designed four treatments with six replicates per treatment according to completely random single factors.The cellulase,xylanase,amylase,andβ-glucanase activities were≥60000,≥2500,≥300000,and≥300000 U·g^(−1) of the silage cellulase used in the experiment.These factors were added to alfalfa silage in four different treatment concentrations:0(T1),2.5‰(T2),5.0‰(T3),and 7.5‰(T4).The results showed that after 60 days,the supplementation of the silage-specific compound enzyme improved the content of crude protein content in group T4(P<0.05).The contents of acidic detergent fiber in groups T3 and T4 were significantly lower than those in group T1(P<0.01),whereas the relative feeding values of T3 and T4 were significantly higher than those of T1(P<0.01).The pH of the silage significantly reduced on adding cellulases(P<0.01);the lactic acid and lactic acid/acetic acid contents of groups T2 and T3 were significantly higher than those of group T1(P<0.05),and the stable aerobic exposure times of T2 and T3 were significantly lower than those of T1 and T4(P<0.05).In summary,under the conditions of this study,the silage-specific cellulase improved the fermentation quality and nutritional value of alfalfa silage,and the optimum supplementation level of silage-specific cellulase was 5.0‰.

关 键 词：苜蓿青贮 营养成分 发酵品质 微生物 有氧稳定性 复合纤维素酶 V-score 

分 类 号：S816.53[农业科学—饲料科学]