余甘子中有效成分与矿质元素含量的相关性研究  

Study on Correlation between Effective Components and Mineral Element Content in Phyllanthus emblica

作　　者：杨晓琼 何璐[1,2] 袁建民 许智萍[1,2] 孔维喜 雷虓 YANG Xiao-qiong;HE Lu;YUAN Jian-min;XU Zhi-ping;KONG Wei-xi;LEI Xiao(Institute of Tropical Eco-Agriculture Sciences,Yunnan Academy of Agricultural Sciences,Yuanmou 651300,China;Yuanmou Dry-Hot Valley Botanical Garden,Yuanmou 651300,China)

机构地区：[1]云南省农业科学院热区生态农业研究所,云南元谋651300 [2]云南省元谋干热河谷植物园,云南元谋651300

出　　处：《江西农业学报》2021年第7期38-47,共10页Acta Agriculturae Jiangxi

基　　金：国家重点研发计划项目“西南干旱河谷区生态综合治理及生态产业发展技术研发第六课题:西南干旱河谷特色生物资源利用技术研发”;特色植物资源罗望子与余甘子野生资源价值评估及品种培育(2017YFC0505106-1)。

摘　　要：利用高效液相色谱法分析了18种余甘子中有效成分没食子酸的含量,采用ICP-OES(电感耦合等离子体发射光谱)法测定了20种矿质元素(Fe、Al、B、Cr、Cu、Mn、Zn、Ca、K、Na、P、S、Se、Si、Mo、Mg、Cd、Co、Ni、Pb)的含量,并对没食子酸与矿质元素含量进行了相关性分析。结果表明:当色谱分析中的洗脱液为乙腈+0.2%磷酸水(5∶95)时,没食子酸浓度(x)与峰面积(y)间的线性回归方程为y=18934x-16.921,R^(2)=0.9999;在18种余甘子中,BCYY的没食子酸含量最高,达(3011.589±34.368)mg/kg;在20种矿质元素中,K、Mg、Ca、S和P含量较高,而Ni、Cr含量较低;没食子酸含量与Ca含量呈极显著负相关,与Na含量呈显著的负相关,说明余甘子中有效成分与矿质元素含量间存在一定的相关性。In this study,the content of gallic acid in 18 species of Phyllanthus emblica was determined by HPLC,and 20 mineral elements(Fe,Al,B,Cr,Cu,Mn,Zn,Ca,K,Na,P,S,Se,Si,Mo,Mg,Cd,Co,Ni and Pb)were determined by ICP-OES(inductively coupled plasma emission spectrometry).The correlation between gallic acid and mineral elements was analyzed.The results showed that when the chromatographic condition was acetonitrile+0.2% phosphoric water(5∶95),the linear regression equation was y=18934 x-16.921 and R^(2)=0.9999,and the gallic acid content in BCYY was the highest,reaching(3011.589±34.368)mg/kg.Among the 20 mineral elements in 18 species of Phyllanthus emblica,K,Mg,Ca,S and P were the richest,while Ni and Cr were the lowest.The content of gallic acid was significantly negatively correlated with the content of Ca,and significantly negatively correlated with the content of Na,which indicated that there was a certain correlation between effective components and mineral element content in Phyllanthus emblica.

关 键 词：余甘子 没食子酸 ICP-OES 矿质元素 相关性分析 

分 类 号：S567.19[农业科学—中草药栽培]