外源性L-抗坏血酸对斑玉蕈子实体发育过程中氧化还原体系的影响  

The effect of exogenous L-ascoorbic acid on the redox system during the development of the fruit body of Hypsizygus marmoreus

作　　者：戴建成 陈辉[2] 张津京[2] 郝海波 韩灿灿 赵明文[1] 冯志勇[1,2] DAI Jiancheng;CHEN Hui;ZHANG Jinjing;HAO Haibo;HAN Cancan;ZHAO Mingwen;FENG Zhiyong(College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China;Shanghai Key Laboratory of Agricultural Genetics and Breeding/Key Laboratory of Resources and Utilization of Edible Fungi(South),Ministry of Agriculture and Rural Affairs/National Engineering Research Center of Edible Fungi,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]南京农业大学生命科学学院,江苏南京210095 [2]上海市农业科学院食用菌研究所上海市农业遗传育种重点开放实验室/农业农村部南方食用菌资源利用重点实验室/国家食用菌工程技术研究中心,上海201403

出　　处：《南京农业大学学报》2021年第4期686-694,共9页Journal of Nanjing Agricultural University

基　　金：上海市食用菌产业技术体系建设专项资金(沪农科产字〔2021〕第9号);国家自然科学基金青年基金项目(31601802,31401932)。

摘　　要：[目的]在不同培养时期的斑玉蕈菌丝中添加外源L-抗坏血酸,探究外源L-抗坏血酸对斑玉蕈产量和子实体发育阶段的氧化还原体系(抗氧化物质、活性氧和抗氧化酶)的影响。[方法]以工厂化生产的斑玉蕈菌株作为研究材料,将培养时间为40和120 d的菌丝搔菌后,处理组分别添加15 mL 400 mg·L^(-1) L-抗坏血酸(AsA)。空白对照组则添加15 mL蒸馏水。测量斑玉蕈子实体的产量和斑玉蕈恢复期(H-M)、转色期(H-V)、原基期(H-P)和成熟子实体期(H-F)的抗氧化物质、活性氧含量,分析抗氧化相关酶活性的变化规律。[结果]培养40 d时,外源添加400 mg·L^(^(-1)) AsA处理组斑玉蕈产量为每瓶104.01 g,比对照组增加10%;培养120 d时,处理组斑玉蕈产量为每瓶278.49 g,比对照组增加5%。外源添加AsA能调控斑玉蕈内源AsA和还原型谷胱甘肽(GSH)含量,培养40 d H-V的内源AsA和GSH含量与120 d的H-M内源AsA和GSH含量均显著提高;培养120 d氧化型谷胱甘肽(GSSG)含量比40 d高。培养120 d菌丝中丙二醛和活性氧含量比40 d高。外源添加的AsA在调控40和120 d的作用时期也不一致,其对40 d的子实体在发育后期(H-P和H-F)作用显著,而对120 d的子实体则在发育早期(H-M和H-V)作用显著;其对抗氧化酶的作用时期和活性变化也不同,其中谷胱甘肽过氧化物酶(GPX)活性差异最大,即在40 d GPX活性呈逐渐上升趋势,在120 d GPX活性呈逐渐下降趋势。[结论]外源添加AsA能调控斑玉蕈内源AsA的分泌规律,提高抗氧化酶活性,减少活性氧累积和脂质过氧化,从而增加子实体产量。[Objectives]The paper aimed to explore the effects of exogenous L-ascorbic acid(AsA)on the yield and the redox system(antioxidants,reactive oxygen species and antioxidant enzymes)during the development of fruiting body of ostreatus maculatus by adding exogenous L-ascorbic acid to the mycelium of Hypsizygus marmoreus at different culture stages.[Methods]The mycelia of Hypsizygus marmoreus was cultured for 40 and 120 d,and then the treatment group was added with the 15 mL of 400 mg·L^(-1)AsA and the control group was added 15 mL distilled water.The yield of fruiting body,the contents of antioxidant substances and reactive oxygen species in the mycelial regeneration stage(H-M),mycelial pigmentation stage(H-V),primordium stage(H-P)and fruiting body stage(H-F)were measured,and the changes of antioxidant enzyme activities were analyzed.[Results]At 40 d,the production of Hypsizygus marmoreus in the 400 mg·L^(-1) AsA treatment group was 104.01 g,which was 10%higher than the control group;at 120 d,the production of Hypsizygus marmoreus in the treatment group was 278.49 g,which was 5%higher than the control group.In addition,exogenous AsA could regulate the contents of endogenous AsA and reduced glutathione(GSH),and the contents of endogenous AsA and GSH at H-V of 40 d were significantly higher than those at 120 d of H-M;the secretion of oxidized glutathione(GSSG)content was similar in 40 and 120 d,but the content of GSSG at 120 d was higher than that at 40 d.The content of malondialdehyde(MDA)and reactive oxygen species(ROS)in mycelia of 120 d were higher than these of 40 d.The effect period of exogenous ASA at 40 and 120 d was also different.It had significant effect on the late development(H-P and H-F)of 40 d fruiting body,but it had significant effect on the early development(H-M and H-V)of 120 d fruiting body;the action period and activity changes of antioxidant enzymes were also different,among which the difference of glutathione peroxidase(GPX)activity was the largest.GPX activity showed a gradual upward trend at

关 键 词：斑玉蕈 L-抗坏血酸 氧化还原体系 子实体发育 抗氧化酶系 

分 类 号：S759.81[农业科学—森林经理学]