b-FGF、TGF-β1、microRNA-34a与慢性乙型肝炎肝纤维化程度的相关性  

Correlation between b-FGF,TGF-β1,microRNA-34a and the degree of liver fibrosis in patients with chronic hepatitis B

作　　者：李园园 苏峰[2] 王艳 栾兴龙[2] LI Yuanyuan;SU Feng;WANG Yan;LUAN Xinglong(Department of Gastroenterology,Huxi Hospital Affiliated of Jining Medical College,Heze,Shandong,China,274000;Department of Gastroenterology,Shan County Central Hospital,Heze,Shandong,China,274000)

机构地区：[1]济宁医学院附属湖西医院消化内科,山东菏泽274000 [2]单县中心医院消化内科,山东菏泽274000

出　　处：《分子诊断与治疗杂志》2021年第6期1002-1005,共4页Journal of Molecular Diagnostics and Therapy

基　　金：山东省医药卫生科技发展计划项目(2018ws135)。

摘　　要：目的分析血清碱性成纤维细胞生长因子(b-FGF)、转化生长因子-β1(TGF-β1)、微小RNA(miRNA)-34a与慢性乙型肝炎(CHB)肝纤维化的相关性。方法选取2018年9月至2020年9月本院收治的180例CHB患者作为CHB组,依据肝脏硬度测量值(LSM)将患者分为轻度肝纤维化组43例、中度肝纤维化组78例、重度肝纤维化组59例,同时选取170例同期于本院接受健康体检且结果正常者作为对照组。对比各组间b-FGF、TGF-β1及microRNA-34a表达差异,采用Pearson相关性分析b-FGF、TGF-β1及microRNA-34a水平与CHB肝纤维化的相关性。结果 CHB组血清b-FGF、TGF-β1、microRNA-34a水平高于对照组,差异均有统计学意义(P<0.05)。不同肝纤维化程度患者b-FGF、TGF-β1、microRNA-34a水平比较:轻度纤维化组<中度纤维化组<重度纤维化组,差异均有统计学意义(P<0.05)。CHB患者b-FGF、TGF-β1、microRNA-34a水平与LSM呈正相关(P<0.05)。结论 b-FGF、TGF-β1及microRNA-34a表达与CHB纤维化程度呈显著相关性,联合检测三者表达情况对CHB病情和纤维化具有一定的诊断价值。Objective To analyze the correlation between the serum basic fibroblast growth factor(b⁃FGF),transforming growth factor⁃β1(TGF⁃β1)and microRNA(miRNA)⁃34a and the liver fibrosis in patients with chronic hepatitis B(CHB).Methods The 180 CHB patients admitted to this hospital from September 2018 to September 2020 were selected as the CHB group.According to the liver stillness measurement(LSM),the patients were divided into a mild liver fibrosis group with 43 cases and a moderate liver group,and a severe liver fibrosis group with 59 cases.At the same time,170 cases were selected as the control group who received a health checkup in this hospital during the same period.The differences in the levels of b⁃FGF,TGF⁃β1 and microRNA⁃34a among the groups were compared,and the correlation between b⁃FGF,TGF⁃β1 and microRNA⁃34a levels and CHB liver fibrosis was analyzed by Pearson correlation.Results The levels of serum b⁃FGF,TGF⁃β1 and microRNA⁃34a in the CHB group were higher than those in the control group,and the difference was statistically significant(P<0.05).The levels of b⁃FGF,TGF⁃β1 and microRNA⁃34a in patients with different degrees of liver fibrosis were as follows:the mild fibrosis group<the moderate fibrosis group <the severe fibrosis group. The difference was statistically significant(P<0.05). The levels of b⁃FGF,TGF⁃β1 and microRNA⁃34a in CHB patients were positively correlated with LSM,and the difference was statistically significant(P<0.05). Conclusion The expressions of b⁃FGF,TGF⁃β1 and microRNA⁃34a are significantly correlated with the degree of CHB fibrosis. The combined detection of the three expressions has certain diagnostic value for CHB disease and fibrosis.

关 键 词：B-FGF TGF-Β1 microRNA-34a 慢性乙型肝炎 肝纤维化 

分 类 号：R575.2[医药卫生—消化系统] R512.62[医药卫生—内科学]