不同香型烤烟中Amadori化合物测定及多元统计分析  

Determination and multivariate statistics of Amadori compounds in flue-cured tobacco leaves of different flavor types

作　　者：马立超[1] 李登科[1] 张春涛[1] 于洁 魏鹏程[1] 田志章 许峰[1] 吴若昕 邢立霞 田楠 MA Lichao;LI Dengke;ZHANG Chuntao;YU Jie;WEI Pengcheng;TIAN Zhizhang;XU Feng;WU Ruoxin;XING Lixia;TIAN Nan(Tianjin Work Station,Technical Center,Shanghai Tobacco Group Co.,Ltd.,Tianjin 300163,China;Technical Center,Shanghai Tobacco Group Co.,Ltd.,Shanghai 201315,China)

机构地区：[1]上海烟草集团有限责任公司技术中心天津工作站,天津市300163 [2]上海烟草集团有限责任公司技术中心,上海市201315

出　　处：《烟草科技》2021年第7期59-69,共11页Tobacco Science & Technology

基　　金：上海烟草集团有限责任公司科技项目“Maillard反应中间体在卷烟重点工序的变化研究”(K2020-2-002P)。

摘　　要：采用液相色谱-串联质谱(LC-MS/MS)法快速测定了烤烟中11种Amadori化合物,并结合主成分分析(PCA)、层次聚类热力图分析(HCHMA)及t分布随机邻域嵌入(t-SNE)等多元统计分析方法,研究Amadori化合物与烤烟香型的关系。结果表明:①目标化合物的线性关系良好(r>0.995);检出限(LODs)为1.0~10.0 ng/mL;日内和日间精密度(RSDs)分别为1.1%~4.8%和2.9%~9.6%(n=6);回收率为87.8%~110.2%。②PCA和t-SNE分析结果表明不同香型烤烟具有明显的分类趋势,其中t-SNE分析的区分效果更明显;HCHMA将样品划分为8类,与香型和产地对应较好。③Amadori化合物测定结果结合多元统计分析方法,可为烤烟香型和产地分析、烟叶品质评价、配方设计优化提供技术支持。A rapid analytical method based on liquid chromatography-tandem mass spectrometry was developed for simultaneous determining 11 Amadori compounds in flue-cured tobacco leaves.The relationships between the Amadori compounds and flavor types of flue-cured tobacco were studied by using multivariate statistic analysis including principal component analysis(PCA),hierarchical clustering heat map analysis(HCHMA)and t-distributed stochastic neighbor embedding(t-SNE).The results showed that:1)There were good linear relationships for the 11 Amadori compounds with the correlation coefficients(r)higher than 0.995.The limits of detection(LODs)were from 1.0 to 10.0 ng/mL,the intra-and inter-day precisions(RSDs)were 1.1%-4.8% and 2.9%-9.6%(n=6)respectively,and the spiked recoveries of samples ranged from 87.8%to 110.2%.2)The results of PCA and t-SNE suggested that there was a clear classification trend for flue-cured tobacco of different flavor types,especially t-SNE analysis.Via HCHMA,all the samples could be classified into eight clusters which corresponded to eight flavor types and were consistent with their production areas.3)The determination and multivariate statistics of Amadori compounds provided technical insights into the flavor types and their production areas,useful to evaluate tobacco leaf quality and blend design.

关 键 词：烤烟 香型 AMADORI化合物 液相色谱-串联质谱(LC-MS/MS) 主成分分析(PCA) 层次聚类热力图分析(HCHMA) t分布随机邻域嵌入(t-SNE) 

分 类 号：TS411.1[农业科学—烟草工业]