利多卡因抑制ERK信号通路激活降低甲状腺癌细胞TPC-1的增殖能力  

Lidocaine suppresses thyroid cancer cell TPC-1 proliferation via inhibiting activation of ERK signaling pathway

作　　者：吴毅[1] 刘付宁[1] 严颖哲 杨浩杰 谢乾 WU Yi;LIU Fu-ning;YAN Ying-zhe;YANG Hao-jie;XIE Qian(Department of Anesthesiology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;Department of Anesthesiology,The First Affiliated Hospital of Jinan University,Guangzhou 510632,China)

机构地区：[1]中山大学孙逸仙纪念医院麻醉科,广州510120 [2]暨南大学附属第一医院麻醉科,广州510632

出　　处：《岭南现代临床外科》2021年第3期294-297,共4页Lingnan Modern Clinics in Surgery

基　　金：广东省中医药局科研项目(项目编号20211084)。

摘　　要：目的研究利多卡因对甲状腺癌细胞的影响及其作用机制。方法10μM、100μM利多卡因处理甲状腺癌细胞TPC-1后,进行CCK8实验检测细胞增殖,流式细胞学实验检测细胞周期,Western Blot检测细胞周期蛋白变化。BALB/C裸鼠皮下成瘤实验比较利多卡因与对照组对肿瘤生长的影响。结果10μM、100μM利多卡因处理后,CCK8结果显示两种浓度下TPC-1细胞增殖均可被抑制,且100μM利多卡因抑制作用强于10μM。流式细胞学结果显示两种浓度下均有S期细胞比值降低,G0/G1期细胞比值增加。Western Blot结果显示两种浓度下均有p21、p27蛋白表达量升高,ERK与p-ERK表达下调。皮下成瘤实验结果显示利多卡因可抑制TPC-1细胞在裸鼠体内生长。结论利多卡因可以抑制甲状腺癌细胞TPC-1的增殖能力,其作用机制可能与抑制ERK信号通路激活有关。Objective To investigate the effects of lidocaine on thyroid cancer cell and its mechanism.Methods After treating thyroid cancer cell TPC-1 with 10μM or 100μM lidocaine in vitro,the changes of cell proliferation was detected by conducting CCK-8 assay,cell cycle was detected with flow cytometry and cyclin expression was determinged byWestern Blot.In vivo,after subcutaneous xenograft models established in BALB/c nude mice,comparison of the effect of lidocaine on tumor growth was performed between BALB/c nude mice andcontrols.Results After TPC-1 cells treated with 10μM or100μM lidocaine in vitro,CCK-8 assay showed TPC-1 cell proliferation can be suppressed with 100μM exerting stronger effect than 10μM.Flow cytometry showed lidocaine-treated cells were attenuated in phase S while accentuated in phase G0/G1.Western blot showed p21 and p27 expressionswere both upregulated while ERK and p-ERK expressions were both down-regulated in cells treated with lidocaine.Subcutaneous xenograft also showed tumor growth can be suppressed by lidocaine injection in nude mice.Conclusion Lidocaine can suppress proliferation of thyroid cancer cell TPC-1 both in vitro and in vivo.The mechanism could be inhibition of ERK signaling pathway activation.

关 键 词：甲状腺癌 利多卡因 增殖 

分 类 号：R736.1[医药卫生—肿瘤]