NADH regulates the expression of cFos and Npas4 through modulating NMDARs activity  

作　　者：Tan Xu Liu Xiaoyu Li Meng Zhang Yue Song Ruiheng Zhang Jing Gao Qing Sun Jinhao 

机构地区：[1]Department of Anatomy and Neurobiology,School of Basic Medicine,Shandong University,Jinan 250012,China

出　　处：《解剖学杂志》2021年第S01期81-81,共1页Chinese Journal of Anatomy

摘　　要：The transcription and translation of the immediate-early genes(IEGs),such as Arc,Zif268,eFos and Npas4,are considered to be molecular mechanisms underlying learning and memory.Recent studies have shown that application of NADH triggered the expression of Arc and Zif268 by activation of NMDA receptor(NMDAR)signaling.In this regard,we hypothesize that levels of Npas4 and c Fos,could be modulated by NMDAR activity-dependent NADH.To test our hypothesis,in primary cultured hippocampal neurons,we employed NADH to examine the effects of NADH on IEGs.Further more,NMDARs blocker MK801 were used to investigated the role of NMDAR in NADH's effects.The data of real-time PCR revealed that application of NADH promoted the transcription of Arc,cFos and Npas4.Similarly,Western blot analyses showed that the levels of Arc,c Fos and Npas4 were increased significantly after NADH treatment.

关 键 词：CFOS NMDAR MK801 NADH 

分 类 号：R74[医药卫生—神经病学与精神病学]