BAF45D cooperates with SMAD signaling and promotes early neural differentiation in P19 and H9 cells  

作　　者：Hu Xiujuan Jiang Jing Chen Xueying Liu Chang Liu Lihua Fang Shengyun Shen Yuxian Liu Chao 

机构地区：[1]Department of Histology and Embryology,Institute of Stem Cell and Tissue Engineering,School of Basic Medical Sciences [2]Institute of Clinical Phar macology,Anhui Medical University,Hefei 230032 [3]Center for Biomedical Engineering and Technology,University of Maryland,Baltimore,MD 21201,USA

出　　处：《解剖学杂志》2021年第S01期220-220,共1页Chinese Journal of Anatomy

摘　　要：BAF45Dis a member of BAF complex,which may play a crucial role in neural development.PAX6 determines neuroectoderm formation,which is highly level sensitive.BAF45D is required for retinoic acid(RA)induced PAX6 expression.PAX6 expression is also regulated by TGF-beta/SMAD signaling.However,mechanism of such regulation remains elusive.We therefore sought to explore whether BAF45D regulates PAX6 expression through cooperating with TGF-beta/SMAD signaling.Here we identified BAF45D is required for expression of phosphorylated SMAD3 and PAX6 induced by RA.Genome-wide analysis revealed that during RA-induced early neural differentiation,BAF 45D knockdown failed to activate TGF-beta/SMAD signaling and induce expression of STAT3 and SMAD7,two negative regulators of TGF-beta/SMAD signaling.Moreover,in RA treated P19 and H9 cells,BAF45D interacts with BRG1 and phosphorylated SMAD3.

关 键 词：SMAD expression. PAX6 

分 类 号：R73[医药卫生—肿瘤]