Bioinformatic analysis and functional characterization of CFEM proteins in Setosphaeria turcica  

作　　者：WANG Jian-xia LONG Feng ZHU Hang ZHANG Yan WU Jian-ying SHEN Shen DONG Jin-gao HAO Zhi-min 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,College of Life Sciences,Hebei Agricultural University,Baoding 071001,P.R.China [2]College of Plant Protection,Hebei Agricultural University,Baoding 071001,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第9期2438-2449,共12页农业科学学报（英文版）

基　　金：supported by the grants from the Natural Science Foundation of Hebei Province,China(C2020204172 and C2018204120);the China Agriculture Research System of MOF and MARA(CARS-02-25);the National Natural Science Foundation of China(31601598 and 31901827)。

摘　　要：Common in Fungal Extracellular Membrane(CFEM)domains are uniquely found in fungal extracellular membrane proteins which are important for pathogens.This study identified 13 StCFEM proteins in the genome of Setosphaeria turcica,the hemibiotrophic fungus that causes northern corn leaf blight.Sequence alignment and WebLogo analysis of their CFEM domains indicated that the amino acids were highly conserved and that,with the exception of StCFEM1,2,3,and 6,they contained eight cysteines.Phylogenic analysis suggested that these 13 proteins(StCFEM1–13)could be divided into 2 clades based on the presence of the trans-membrane domain.Six StCFEM proteins with a signal peptide and without a trans-membrane domain were considered as candidate effector proteins.The CFEM domain in the candidate effector proteins could form a helical-basket structure homologous to Csa2 in Candida albicans.Transcriptome analysis suggested that the 13 genes were expressed during fungal infection and a yeast secretion assay revealed that these candidate effectors were secreted proteins.It was also found that StCFEM3,4,and 5 couldn't affect BAX/INF1-induced programmed cell death(PCD)in Nicotiana benthamiana and while StCFEM12 could suppress INF1-induced PCD,it showed no effect on BAXinduced PCD.This study found that there were 13 members of the S.turcica CFEM protein family and that StCFEM12 was a candidate effector.This study laid the foundation for illustrating the roles of CFEM proteins during the pathogenic processes of phytopathogens.

关 键 词：CFEM domain EFFECTOR Setosphaeria turcica signal peptide PCD 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]