核受体FXR激活对宫颈癌CaSki细胞增殖的抑制效应及其作用机制  

Inhibitory effects and mechanisms of nuclear receptor FXR activation in cervical cancer cell line CaSki

作　　者：黄晓华 牛永东[2] 石刚刚[2] HUANG Xiaohua;NIU Yongdong;SHI Ganggang(The Second Affiliated Hospital of Shantou University Medical College,Shantou 515041;Shantou University Medical College,Shantou 515041,Guangdong,China)

机构地区：[1]汕头大学医学院第二附属医院,广东汕头515041 [2]汕头大学医学院,广东汕头515041

出　　处：《癌变．畸变．突变》2021年第4期302-306,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省医学科学技术研究基金(2015120104622949)。

摘　　要：目的:探讨激活法尼醇X受体(FXR)对宫颈癌CaSki细胞增殖的抑制效应及其作用机制。方法:用10μmol/L FXR激动剂GW4064处理CaSki细胞,同时以DMSO处理组细胞为对照。分别于处理24和48 h后,采用荧光定量PCR和Western blot检测CaSki细胞FXR mRNA和蛋白的表达情况,用四甲基噻唑蓝(MTT)法检测激活FXR后CaSki细胞的增殖率,流式细胞技术检测细胞凋亡率,Western blot检测p53蛋白的表达水平。结果:与对照组相比,GW4064作用组CaSki细胞FXR mRNA和蛋白的表达水平升高,细胞增殖率降低,细胞凋亡率升高,同时p53蛋白的表达水平升高(均为P<0.05)。结论:激活FXR可抑制CaSki细胞株增殖并促进细胞凋亡,这可能与上调p53表达有关。OBJECTIVE:To investigate inhibitory effects and the mechanisms of farnesoid X receptor(FXR)on proliferation of a cervical cancer cell line,CaSki.METHODS:FXR was activated by the FXR agonist GW4064 and DMSO was set as the control group at the same time.After treatment with 10μmol/L GW4064 for 24 and 48 h,the mRNA and protein levels of FXR were detected by fluorescence quantitative PCR and Western blot,respectively.Proliferations of CaSki were detected by using methylthiazolyldiphenyltetrazolium bromide(MTT)assay after FXR activation.Cell apoptosis of CaSki was detected by flow cytometry.Western blot was used to detect protein levels of p53.RESULTS:Compared with the control group,expression levels of FXR mRNA and protein in the GW4064 treatment group were increased,cell proliferation rates were decreased,apoptosis rates were increased,and expression levels of p53 protein were increased(all P<0.05).CONCLUSION:Activation of FXR inhibited proliferation of CaSki through promoting cell apoptosis which might be related to increased expression of p53.

关 键 词：法尼醇X受体 CASKI细胞 细胞增殖 凋亡 P53 

分 类 号：R737.33[医药卫生—肿瘤]