木犀草素基于JAK/STAT信号通路对SGC7901胃癌细胞的作用  

Effects of luteolin on stomach cancer SGC7901 cell by JAK/STAT signaling pathway

作　　者：杨晓晔[1] 豆发福[1] 李恒[1] YANG Xiao-ye;DOU Fa-fu;LI Heng(Department of Gastrointestinal Surgery,Hanzhong 3201 Hospital,Hanzhong 723000,China)

机构地区：[1]汉中3201医院胃肠外科,陕西汉中723000

出　　处：《中国现代普通外科进展》2021年第7期514-517,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:研究木犀草素基于JAK/STAT信号通路对SGC7901胃癌细胞的作用。方法:体外培养SGC7901细胞,以1、5、10μmol/L的木犀草素作用24 h,空白对照组不做任何处理,溶剂对照组加入0.5%DMSO,MTT法检测木犀草素对SGC7901细胞增殖的影响,流式细胞技术检测木犀草素对SGC7901细胞凋亡的影响,RT-PCR检测木犀草素作用后SGC7901细胞JAK、STAT mRNA表达的变化,Western blot检测木犀草素作用后SGC7901细胞JAK、STAT蛋白表达的变化。结果:MTT检测结果表明,1、5和10μmol/L木犀草素组细胞活性较空白对照组低(P<0.05);流氏细胞技术检测结果表明,1、5和10μmol/L木犀草素组细胞总凋亡率较空白对照组升高(P<0.05);RT-PCR结果表明,1、5和10μmol/L木犀草素组的JAK、STATmRNA表达水平较空白对照组均降低(P<0.05)。Western blot结果表明,1、5、10μmol/L木犀草素组JAK蛋白和STAT蛋白表达水平较空白对照组均降低(P<0.05)。结论:木犀草素可抑制SGC7901细胞增殖、诱导SGC7901细胞凋亡,其作用机制可能与JAK/STAT信号通路有关。Objective: To investigate the effects of luteolin on stomach cancer SGC7901 cell based on JAK/STAT signaling pathway. Methods: SGC7901 cells culture in vitro were treated with 1、5、10 μmol/L concentrations of luteolin for 24 h. The blank control group was not treated. The solvent control group was added with 0.5% DMSO. The effect of luteolin on the proliferation of SGC7901 cell was analyzed byMTT. The effect of luteolin on the apoptosis of SGC7901 cell was analyzed by flow cytometer.The expressions of JAK, STAT mRNA in cells were tested by RT-PCR. The expressions ofJAK, STAT protein in cells were tested by Western blot. Results: Cell proliferate activity in 1, 5 and 10μmol/L luteolin group were lower than normal control group analyzed by MTT(P<0.05). The total rates of cell apoptosis in 1, 5 and 10μmol/L luteolin group were higher than normal control group analyzed by flow cytometer(P<0.05). The expressions of JAK, STAT mRNA in 1, 5 and 10 μmol/L luteolin group were lower than normal control group analyzed by RT-PCR(P<0.05). The expressions of JAK protein and STAT protein in 1, 5 and 10 μmol/L luteolin group were lower than normal control group analyzed byWestern blot(P<0.05). Conclusion: Luteolin may inhibit the proliferation and induce apoptosis of stomach cancer SGC7901 cell. Themechanism may be related to JAK/STATsignaling pathway.

关 键 词：木犀草素 胃肿瘤 SGC7901细胞 JAK-STAT信号通路 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]