耐高温菌株大曲高温放线菌CICC 10681培养条件的响应面优化  

Response surface optimization of the culture conditions of thermotolerant Thermoactinomyces daqus CICC 10681

作　　者：刘红强 于学健 翟磊 白飞荣 姚粟 LIU Hongqiang;YU Xuejian;ZHAI Lei;BAI Feirong;YAO Su(China National Research Institute of Food and Fermentation Industries Co.Ltd.,China Center of Industrial Culture Collection,Beijing 100015,China)

机构地区：[1]中国食品发酵工业研究院有限公司,中国工业微生物菌种保藏管理中心,北京100015

出　　处：《食品与发酵工业》2021年第14期123-129,共7页Food and Fermentation Industries

基　　金：国家重点研发计划资助(2016YFD0400502)。

摘　　要：为了获得耐高温菌株大曲高温放线菌(Thermoactinomyces daqus,T.daqus)CICC 10681的最佳培养条件,以生物量(OD_(620nm))为响应值,通过单因素试验、Plackett-Burman、响应面试验对菌株CICC 10681的生长条件进行优化。研究结果表明,T.daqus CICC 10681的最佳培养条件为可溶性淀粉4.5 g/L、大豆蛋白胨8.4 g/L、硫酸镁5 g/L、53℃、接种量2%、装液量60 mL/250mL、转速180 r/min,在此条件下进行验证试验,菌株OD 620nm达到1.98±0.029,较优化前提高了6.2倍,因此运用响应面试验预测的该模型有一定的实践指导意义,为后续菌株在高温大曲堆积发酵生产中的应用提供理论基础。To obtain the optimal culture conditions of Thermoactinomyces daqus CICC 10681,single-factor test,Plackett-Burman and response surface test were conducted using biomass OD_(620nm) as the response value.The results showed that the optimal culture parameters of T.daqus CICC 10681 were:soluble starch 4.5 g/L,soy peptone 8.4 g/L,MgSO 45 g/L,53℃,inoculation volume 2%,loaded volume 60 mL/250mL and rotate speed at 180 r/min.Under this condition,the OD 620nm value was up to 1.98±0.029,which was 6.2-fold higher than the value before optimization.Therefore,the model has certain practical guidance significance,and provides a theoretical basis for the application of subsequent strains in Daqu accumulation fermentation.

关 键 词：大曲高温放线菌 嗜高温 培养条件 响应面优化 高温酶 

分 类 号：TS261.11[轻工技术与工程—发酵工程]