丹参水溶性组分SABP对小鼠肝癌免疫微环境的影响  

Effect of SABP,A Water-soluble Component of Salvia Miltiorrhiza,on Immune Microenvironment of Mice with Liver Cancer

作　　者：李圣豪 郝立园 国英琳 彭晴 丁静茹 石新丽 LI Shenghao;HAO Liyuan;GUO Yinglin;PENG Qing;DING Jingru;SHI Xinli(Hebei Provincial Key Laboratory of Integrative Medicine for Liver&Kidney Diseases,Shijiazhuang 050091,China;Basic Medical College,Hebei University of Chinese Medicine,Shijiazhuang 050200,China)

机构地区：[1]河北省中西医结合肝肾病证研究重点实验室,石家庄050091 [2]河北中医学院基础医学院,石家庄050200

出　　处：《肿瘤防治研究》2021年第7期694-698,共5页Cancer Research on Prevention and Treatment

基　　金：河北省中西医结合肝肾病证研究重点实验室开放课题(A201904);河北省人才工程培养资助项目(A201902015);河北省高校百名优秀创新人才支持计划项目(SLRC2019043);河北省自然科学基金面上项目(H2020423009);国家自然基金面上项目(81873112);研究生创新能力培养资助项目(XCXZZSS2020001)。

摘　　要：目的探讨丹参水溶性组分SABP影响H22细胞肝癌原位移植瘤生长及肝癌免疫微环境的效应。方法建立H22细胞肝癌原位移植瘤小鼠模型。ELISA检测肝脏中PD-L1、TGF-β、IL-1β、IL-10、IL-4、IFN-γ、IL-18、IL-7、IL-2、CCL-2和CCL-21的表达量。计算肝脏、脾脏和肾脏的脏器系数。结果SABP抑制H22细胞肝癌原位移植瘤的生长,提高肝癌微环境中PD-L1、TGF-β、IL-1β和IL-10的表达水平,增加了肝、脾和肾系数。结论SABP能抑制H22细胞肝癌原位移植瘤生长,促进肝癌微环境中PD-L1、TGF-β、IL-1β和IL-10抑制性免疫分子的表达。Objective To investigate the effect of SABP,a water-soluble component of Salvia miltiorrhiza,on the growth of orthotopic transplantation of H22 liver cancer and the immune microenvironment of liver cancer.Methods We established a mouse model of orthotopic transplantation of H22 cell liver cancer in BALB/c mice.ELISA was used to detect the expression of PD-L1,TGF-β,IL-1β,IL-10,IL-4,IFN-γ,IL-18,IL-7,IL-2,CCL-2 and CCL-21 in the liver.We counted the organ indexes of liver,spleen and kidney.Results SABP inhibited the growth of orthotopic transplantation tumors of H22 cell liver cancer,and increased the expression levels of PD-L1,TGF-β,IL-1βand IL-10 in the microenvironment of liver cancer,as well as the liver,spleen and kidney coefficients.Conclusion SABP could inhibit the growth of orthotopic transplantation tumors of H22 cell liver cancer and promote the expression of PD-L1,TGF-β,IL-1βand IL-10 in the microenvironment of liver cancer.

关 键 词：丹参 水溶组分 肝癌 免疫微环境 原位移植瘤 

分 类 号：R735.7[医药卫生—肿瘤]