氟他胺对前列腺癌细胞LNCaP线粒体呼吸链酶学变化的影响  

Effects of enzyme on the mitochondrial respiratory chain in prostate cancer cells LNCaP by flu-tamide

作　　者：谢蛟魁 宋印娥 陈海文[2] 陈琦[2] 田宏[3] Xie Jiaokui;Song Yine;Chen Haiwen;Chen Qi;Tian Hong(Department of Urology,Ankang Central Hospital,Ankang 725000,China;Department of Urology,the Second Affiliated Hospital of Medical School of Xi'an Jiaotong University,Xi'an 710004,China;The Research Center of Reproductive Medicine of Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]安康市中心医院泌尿外科,安康725000 [2]西安交通大学第二附属医院泌尿外科,西安710004 [3]西安交通大学生殖医学研究中心,西安710061

出　　处：《国际泌尿系统杂志》2021年第4期607-611,共5页International Journal of Urology and Nephrology

摘　　要：目的观察氟他胺(FLU)对人前列腺癌细胞LNCaP细胞株线粒体呼吸链酶学变化的影响,探讨FLU引起LNCaP细胞株线粒体呼吸链酶学变化的作用机制。方法获得雄激素依赖性细胞(LNCaP)细胞株,进行细胞培养,获得稳定生长及传代的细胞株。不同浓度的雄激素受体阻滞剂FLU作用于培养的LNCaP细胞株;不同时间点提取干预措施下的LNCaP细胞株线粒体;检测并比较不同时间点、不同浓度药物作用下LNCaP细胞株线粒体呼吸链酶学的变化和差异。结果FLU作用于LNCaP细胞株后,形态学活力逐渐减弱,提取三种剂量组FLU作用于LNCaP细胞株后,在不同时间点提取线粒体,实测数据及统计线图均显示:其蛋白的含量增加显著(P<0.05),但琥珀酸脱氢酶(SDH)、烟酰胺腺嘌呤二核苷酸(NADH)、细胞色素C氧化酶(C0X)的含量显著下降(P<0.05),各个指标在不同的时间点上下降的程度有所差异。结论FLU可引起LNCaP细胞株线粒体内蛋白的表达量下降,抑制LNCaP的增殖。Objective To explore the effects of flutamide(FLU)on the mitochondrial respira-tory chain in prostate cancer cells LNCaP,and to investigate its mechanisms.Methods Androgen-dependent cell lines(LNCaP)were obtained for cell culture to obtain stable growth and passage of cell lines Changes in mitochondrial protein,enzyme changes,the androgen receptor antagonist FLU roles in the culture prostate cancer cell line of androgen at different concentrations drugs.Mitochondria at different time points of LNCaP under the interventions were extracte.Mitochondrial respiratory changes and differences at different time points and under the action of different drug concentration in LNCaP were measured and compared.Results The morphological activity of LNCaP cells was gradually decreased after treated with FLU,After FLU acts on LNCaP cells,The content of mitochondrial protein increased signific antly(P<0.05),hut the content of SDH 、NADH、COX decreased significantly(P<0.05),The decreasing degree of each index is difference in different time points.Conclusions F LU can cause expression of mitochondrial protein decreased in LNCaP,and control cell proliferation.

关 键 词：前列腺肿瘤 细胞系 氟他胺 线粒体 

分 类 号：R737.25[医药卫生—肿瘤]