陆地棉GhMYB4基因沉默载体构建及转基因棉花的鉴定  

Construction of Silencing Vector of GhMYB4 Gene and Identification of Transgenic Cotton

作　　者：王怡[1] 于月华[1] 刘晨 万会娜 王萍 唐洁 倪志勇[1] WangYi;YuYuehua;Liu Chen;Wan Huina;Wang Ping;TangJie;Ni Zhiyong(College of Agriculture,Xinjiang Agricultural University,Urumqi,830052)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052

出　　处：《分子植物育种》2021年第13期4386-4391,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(U1703115);自治区天山创新团队计划(2020D14002);新疆维吾尔自治区自然科学基金面上项目(2018D01A10);自治区天山青年计划(2018Q002;2018Q18)共同资助。

摘　　要：MYB转录因子是植物中最大的转录家族之一,广泛参与植物逆境胁迫应答、生长发育及细胞形态的建成等生理活动。为了研究GhMYB4基因在棉花中的生物学功能,本研究利用重叠PCR方法替换拟南芥At-MIR319的成熟链和互补链序列,构建含有amiRMYB4前体的植物表达载体amiRGhMYB4-pCAMBIA3301,通过农杆菌介导喷花法转化棉花植株。Bar基因PCR检测获得19株阳性植株,319-3301引物PCR检测获得16株阳性植株,3301通用引物PCR检测获得7株阳性植株,综合3组引物检测结果共获得7株阳性植株,以上结果表明,已获得GhMYB4转基因棉花。本研究结果为进一步研究陆地棉GhMYB4基因棉花纤维发育机制提供材料基础。MYB transcription facto rs are one of the largest transcription families in plants.They are widely involved in plant stress response,growth and development,and cell morphogenesis.To study the biological function of GhMYB4 gene in cotton,this study used overlapping PCR method to replace mature and complementary chain sequences of Arabidopsis At-MIR319 to construct plant expression vector amiRGhMYB4-pCAMBIA3301 containing amiRMYB4 precursors,transformation of cotton plants by Agrobacterium tumefaciens-mediated spraying.Bar gene PCR obtained 19 positive plants,319-3301 primer PCR obtained 16 positive plants,3301 universal primer PCR obtained 7 positive plants and 3 primers obtained a total of 7 positive plants.GhMYB4 transgenic cotton has been obtained from the above results.It provides a theoretical basis for further study on the fiber development mechanism of upland cotton GhMYB4 gene.

关 键 词：陆地棉(Gossypium hirsutum) 重叠PCR 棉花转化 

分 类 号：S562[农业科学—作物学]