侧耳木霉产漆酶酶活力测定和脱色方法  

Enzyme Activity and Decolorization of Laccase from Trichoderma pleuroticola

作　　者：张宇婷 宋明珠 王雪峰 刘忠军[1] ZHANG Yuting;SONG Mingzhu;WANG Xuefeng;LIU Zhongjun(National Engineering Research Center of Ginseng,Jilin Agricultural University,Changchun 130118,China;SEMFEED Group,Dalian 116000,China)

机构地区：[1]吉林农业大学国家人参工程研究中心,长春130018 [2]大连塞姆生物工程技术有限公司,大连116000

出　　处：《吉林农业大学学报》2021年第3期317-323,共7页Journal of Jilin Agricultural University

基　　金：吉林农业大学科研启动基金资助项目(201632);吉林省教育厅“十三五”科学技术项目(JJKH20190920KJ);教育部中西部高等学校青年骨干教师国内访问学者项目(2018年)。

摘　　要：为研究木霉粗酶液胞外分泌物的生物活性,从愈创木酚的用量、反应温度、缓冲液种类、缓冲液pH、缓冲液浓度5个因素,对木霉粗酶液胞外分泌物漆酶的酶活力进行研究;从反应时间、反应温度、缓冲液pH、染料浓度、缓冲液浓度、酶液稀释倍数6个因素对木霉粗酶液的胞外分泌物漆酶对染料的脱色作用进行研究。结果表明:木霉漆酶在反应温度为25℃,琥珀酸缓冲液的pH为4.0,琥珀酸缓冲液浓度为0.045 mol/L,愈创木酚用量为0.04 mol/L时,木霉粗酶液胞外分泌物漆酶酶活力最优;木霉粗酶液的胞外分泌物漆酶其脱色作用在反应温度为30℃,pH为3.5,染料浓度为0.01 g/L,醋酸缓冲液浓度为0.2 mol/L时,木霉粗酶液的胞外分泌物漆酶脱色作用最好。In order to study the biological activity of extracellular secretion of Trichoderma crude enzyme solution, enzyme activity of laccase from the extracellular secretion of Trichoderma crude enzyme was studied from five factors including dosage of guaiacol, reaction temperature, type of buffer solution, pH of buffer solution and concentration of buffer solution. Meanwhile, decolorization effect of laccase from extracellular secretion of Trichoderma crude enzyme solution was studied from six factors including reaction time, reaction temperature, buffer pH, dye concentration, buffer concentration and dilution ratio of enzyme solution. The results showed that when reaction temperature was 25 ℃, pH value of succinic acid buffer was 4.0, concentration of succinic acid buffer was 0.045 mol/L, and dosage of guaiacol was 0.04 mol/L, laccase activity of extracellular secretion of Trichoderma crude enzyme was the best;when reaction temperature was 30 ℃, pH value was 3.5,concentration of dye was 0.01 g/L, and concentration of acetic acid buffer was 0.2 mol/L, decolorization effect of laccase from extracellular secretion of Trichoderma crude enzyme was the best.

关 键 词：木霉 漆酶 酶活力 脱色作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q949.325[农业科学—植物保护]