Distinct Characteristics of Odor-evoked Calcium and Electrophysiological Signals in Mitral/Tufted Cells in the Mouse Olfactory Bulb  

作　　者：Han Xu Chi Geng Xinzhong Hua Penglai Liu Jinshan Xu Anan Li 

机构地区：[1]Jiangsu Key Laboratory of Brain Disease and Bioinformation,Research Center for Biochemistry and Molecular Biology,Xuzhou Medical University,Xuzhou 221004,China [2]School of Medical Information and Engineering,Xuzhou Medical University,Xuzhou 221004,China

出　　处：《Neuroscience Bulletin》2021年第7期959-972,共14页神经科学通报（英文版）

基　　金：the National Natural Science Foundation of China(31872771,32070995,and 31900713);the National Demonstration Center for Experimental Basic Medical Science Education(Xuzhou Medical University).

摘　　要：Fiber photometry is a recently-developed method that indirectly measures neural activity by monitoring Ca^(2+)signals in genetically-identified neuronal populations.Although fiber photometry is widely used in neuroscience research,the relationship between the recorded Ca^(2+)signals and direct electrophysiological measurements of neural activity remains elusive.Here,we simultaneously recorded odor-evoked Ca^(2+)and electrophysiological signals[single-unit spikes and local field potentials(LFPs)]from mitral/tufted cells in the olfactory bulb of awake,head-fixed mice.Odors evoked responses in all types of signal but the response characteristics(e.g.,type of response and time course)differed.The Ca^(2+)signal was correlated most closely with power in theβ-band of the LFP.The Ca^(2+)signal performed slightly better at odor classification than high-γoscillations,worse than single-unit spikes,and similarly toβoscillations.These results provide new information to help researchers select an appropriate method for monitoring neural activity under specific conditions.

关 键 词：Mitral/tufted cells Fiber photometry ELECTROPHYSIOLOGY Odor representation Olfactory bulb 

分 类 号：R741[医药卫生—神经病学与精神病学]