菜薹花枝离体培养留种技术研究  

Study on the Seed Reservation Technology by Flowering Branches of Brassica Campestris in Vitro Culture

作　　者：吴艺飞[1] 周晓波[1] 张竹青[1] 刘志全 丁茁荑[1] WUYi-fei;ZHOU Xiao-bo;ZHANG Zhu-qing;LIU Zhi-quan;DING Zhuo-yi(Hunan Vegetable Research Institute,Hunan Vegetable Research and Development Center,Changsha 410125,PRC)

机构地区：[1]湖南省蔬菜研究所,湖南省蔬菜工程技术研究中心,湖南长沙410125

出　　处：《湖南农业科学》2021年第6期1-4,共4页Hunan Agricultural Sciences

基　　金：湖南省自科基金项目(2019JJ0331);湖南省重点研发计划(2018NK2022,2020NK2044);国家大宗蔬菜产业技术体系长沙综合试验站(CARS-23-G-29);湖南省农业科技创新资金项目(2020CX19)。

摘　　要：为了保证秋冬季低温寡照等不良条件下菜薹种子能正常成熟,从而提高菜薹的育种效率,研究剪取菜薹花枝扦插于特定营养液中,从营养液成分、培养条件等方面入手,探讨最适宜的花枝离体培养条件。结果表明:采用10%多抗霉素1 000倍液+0.1%农用链霉素+2%白糖+ 6-BA 2 mg/L的培养液,将扦插花枝置于25-28℃、日照10 h/d的光照培养箱中培养,对菜薹花枝生长最为有利,花枝不易滋生霉菌,可以正常吸收营养以供种子膨大发育成熟,成熟后种子饱满,发芽率可达98.2%。该花枝离体培养留种技术操作简便,有利于菜薹科研工作的顺利开展。In order to ensure the normal maturity of Brassica campestris L.ssp.chinensis var.perperea seeds under adverse conditions such as low temperature and low illumination in autumn and winter,so as to improve the breeding efficiency of Brassica campestris L.ssp.chinensis var.perperea,its flower branches were cut and put into specific nutrient solution to explore the optimal in vitro culture conditions from the aspects of nutrient solution composition and culture conditions.The results showed that using 10%polyoxin 1000 times liquid+0.1%streptomycin+2%sugar+6-BA 2 mg/L as culture medium,the cuttings of flower branches were placed into the light incubator,maintaining sunshine 10 hours/day at 25-28°C,which was the most favorable for the growth of flower branches,so the flower branches were not easy to breed molds and could absorb nutrients normally for seed expansion and maturation.The seeds were plump after ripening and the germination rate was 98.2%.The technique of flower branch in vitro culture for seed reservation is simple and convenient,which is conducive to the smooth development of scientific research work of Brassica campestris L.ssp.chinensis var.perperea.

关 键 词：菜薹 花枝扦插 离体培养 留种技术 

分 类 号：S634.5[农业科学—蔬菜学]