IGF1R反义非编码RNA在肝癌中的差异化表达  

Differential expression of IGF1R antisense noncoding RNA in hepatocellular carcinoma

作　　者：谢志海 周志聪 桂元 孙静锋[3] Xie Zhihai;Zhou Zhicong;Gui Yuan;Sun Jingfeng(Geriatric Disease Treatment Center of Guangzhou Nursing Home, Guangzhou 510000, China;Department of Hepatobiliary and Pancreatic Surgery, Jiangsu Cancer Hospital, Nanjing 210013, China;Department of Oncology, the Fourth Affiliated Hospital of Nanjing Medical University, Nanjing 210013, China)

机构地区：[1]广州市老人院老年病治疗中心,广州510000 [2]江苏省肿瘤医院肝胆胰外科,南京210013 [3]南京医科大学第四附属医院肿瘤外科,南京210013

出　　处：《临床荟萃》2021年第7期620-622,共3页Clinical Focus

基　　金：中国肝炎防治基金会天晴肝病研究基金IRAIN在肝癌演进中的作用(TQGB20200002)。

摘　　要：目的检测胰岛素样生长因子1受体(IGF1R)反义非编码RNA(IGF1RAIN RNA)在肝癌及正常肝组织中的差异,验证其作为肝癌特征性标记物的可能。方法逆转录聚合酶链反应(RT-PCR)法检测IGF1RAIN RNA在30例肝癌癌瘤组织、癌旁组织以及16例肝血管瘤及肝囊肿术后切缘旁正常肝组织中的表达;结合患者临床及病理资料,分析其含量和肝癌的关系。结果肝癌癌瘤中IGF1RAIN RNA含量明显比癌旁及正常肝组织低(P<0.05);不同癌瘤大小、癌瘤分化程度以及肝癌肿瘤病理学分期患者IGF1RAIN RNA表达存在差异(P<0.05)。结论IGF1RAIN RNA的含量在肝癌的明显降低,它可能是一种肝癌早期诊断的标记物。Objective To detect the difference of insulin-like growth factor 1 receptor(IGF1R)antisense noncoding RNA(IGF1RAIN RNA)content between hepatocellular carcinoma and normal hepatic tissues,and to verify its possibility as a characteristic marker of hepatocellular carcinoma.Methods Reverse transcriptase polymerase chain reaction(RT-PCR)was used to detect the expression of IGF1RAIN RNA in 30 cases of hepatocellular carcinoma tissues,adjacent tissues and 16 cases of normal hepatic tissues(adjacent to the resection margin of hepatic hemangioma and hepatic cyst).Combined with the clinical and pathological data,the relationship between the content and hepatocellular cancer was analyzed.Results The content of IGF1RAIN RNA was significantly lower in hepatocellular cancer than that in normal hepatic tissues and adjacent tissues(P<0.05);IGF1RAIN RNA was different in tumor size,tumor differentiation and pathological stage(P<0.05).Conclusion The content of IGF1RAIN RNA was significantly decreased in hepatocellular cancer.It may be a marker for early diagnosis of hepatocellular cancer.

关 键 词：肝肿瘤 生长调节素类 逆转录聚合酶链反应 RNA 

分 类 号：R735.7[医药卫生—肿瘤]