基于微量元素分析的三七产地及其主侧根鉴别  

Discriminant analysis of origin of Panax notoginseng and its main roots and lateral roots based on trace element analysis

作　　者：张棚 张希 杨雪妍 刘元林[1,2] 李儒 龙鸣 田晓静 张福梅[2] 陈士恩 马忠仁[1,2] ZHANG Peng;ZHANG Xi;YANG Xueyan;LIU Yuanlin;LI Ru;LONG Ming;TIAN Xiaojing;ZHANG Fumei;CHEN Shien;MA Zhongren(College of Life Science and Engineering, Northwestern Minzu University, Lanzhou 730124,China;China-Malaysia National Joint Laboratory, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China;College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China;Integrated Technology Center of Lanzhou Customs,Lanzhou 730100, China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730124 [2]西北民族大学生物医学研究中心中国—马来西亚国家联合实验室,甘肃兰州730030 [3]云南中医药大学中药学院,云南昆明650500 [4]兰州海关综合技术中心,甘肃兰州730100

出　　处：《浙江农业学报》2021年第7期1300-1308,共9页Acta Agriculturae Zhejiangensis

基　　金：科技部援助项目(KY201501005);甘肃省科技计划(18JR3RA371);西北民族大学武陵山片区精准扶贫科研项目(31920180001);西北民族大学引进人才科研项目(xbmuyjrc201408)。

摘　　要：为解决三七主侧根粉掺杂、以侧充主等问题,利用干法消解结合电感耦合等离子体发射光谱仪(ICP-OES)技术建立三七中Se、Cd等11种元素同步检测的方法,各元素加标回收率范围在95.72%~102.3%,相关系数r值达0.97198~0.99997,其RSD值为0.09%~2.88%、且均小于3%。分析云南5个不同产地三七及其主侧根中元素含量差异,结果显示:三七中As、Pb、Cd重金属元素含量≤0.5 mg·kg^(-1),均未超标;因子分析(FA)表明,样品元素主要影响因子为As、Fe、Pb;主成分分析(PCA)、典则判别分析(CDA)建立的3D、2D数学模型能够明显区分5种不同产地三七及其主侧根;聚类分析(CA)树状图分类结果与实际产地相符,系统地把不同产地主侧根分为两类,与PCA、CDA分析结果均有一致性。本研究旨为三七产地判别、主根与侧根鉴别提供了快速、准确的新方法。In this study,in order to solve the problems of main and lateral root doping of Panax notoginseng,the method of simultaneous detection of 11 elements such as Se and Cd in Panax notoginseng was established by dry digestion combined with inductively coupled plasma optical emission spectrometry(ICP-OES)technology.The range was from 95.72%to 102.3%,the correlation coefficient r value was 0.97198 to 0.99997,the RSD value were 0.09%to 2.88%,and all were less than 3%.Under this condition,the five different origins of Panax notoginseng and their difference in element content in main and lateral roots were detected in Yunnan.The results showed that the content of As,Pb and Cd in Panax notoginseng was not more than 0.5 mg·kg^(-1);none of them exceeded the standard.FA analysis showed that the main influencing factors of the sample elements were As,Fe and Pb.The 3D and 2D mathematical models established by principal component analysis(PCA)and canonical discriminant analysis(CDA)made a clear distinction between Panax notoginseng and its main roots in 5 different production areas,and there was no overlap.The clustering analysis(CA)results were consistent with the actual production areas,and the main roots of different production areas were systematically divided into two categories.PCA and CDA analysis results were consistent.The purpose of this study was to provide a fast and accurate new method for judging the origin of Panax notoginseng and identifying the main roots and lateral roots.

关 键 词：三七 微量元素 电感耦合等离子体发射光谱仪(ICP-OES) 产地判别 主成分分析 聚类分析 

分 类 号：S567.236[农业科学—中草药栽培]