N-乙酰半胱氨酸对佐剂性关节炎大鼠治疗作用及机制  

Effect and mechanism of N⁃acetylcysteine on adjuvant arthritis rats

作　　者：杨珺[1] 潘献柱[1] 汪晓庆[1] 谢琳琳[1] 刘梅梅[1] 赵大海[2] 叶静[2] 汪陶荣 陈晓宇[3] YANG Jun;PAN Xian-zhu;WANG Xiao-qin;XIE Lin-lin;LIU Mei-mei;ZHAO Da-hai;YE Jing;WANG Tao-rong;CHEN Xiao-yu(Department of Human Anatomy,Anhui Medical College,Hefei 230061,China;Department of Respiratory Medicine,the Second Affiliated Hospital of Anhui Medical University,Hefei 230601,China;Experimental Center of Morphology,Anhui Medical University,Hefei 230032,China)

机构地区：[1]安徽医学高等专科学校形态学实验中心,安徽合肥230061 [2]安徽医科大学第二附属医院呼吸内科,安徽合肥230601 [3]安徽医科大学形态学实验中心,安徽合肥230032

出　　处：《海南医学院学报》2021年第14期1047-1051,1058,共6页Journal of Hainan Medical University

基　　金：安徽省高校自然科学研究重点项目(KJ2019A1100);安徽医科大学基础与临床合作提升计划项目(2019xkj T027)。

摘　　要：目的:探讨N-乙酰半胱氨酸(NAC)对佐剂性关节炎(AA)大鼠的抗炎作用,以及Toll样受体2(TLR2)炎症因子的改变。方法:SD大鼠50只,随机取10只为对照组,40只进行AA造模,造模10 d后,再均分为模型组及NAC低、中、高剂量组(灌胃给药量50、100、200 mg/kg),NAC组给药16 d,应用关节肿胀度和关节炎指数评价NAC对AA大鼠治疗作用;免疫组化染色和Western blot观察关节滑膜组织中TLR2蛋白表达;ELISA法检测大鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)水平。结果:与对照组比较,模型组关节肿胀、关节炎指数明显升高,NAC低、中、高剂量组大鼠关节肿胀和关节炎指数降低明显,差异均具有统计学意义(P<0.05或P<0.01);NAC低、中、高剂量组滑膜组织TLR2蛋白表达减弱,炎症因子TNF-α、IL-1β下降,IL-10上升,差异具有统计学意义(P<0.01)。结论:NAC可改善AA大鼠关节炎症状,其机制可能与抑制AA大鼠TLR2和调节下游炎症因子通路有关。Objective:To investigate the anti⁃inflammatory effects of N⁃acetylcysteine(NAC)on adjuvant arthritis(AA)rats and the changes of Toll⁃like receptor 2(TLR2)⁃inflammatory factors.Methods:50 SD rats were randomly divided into the control group(n=10)and AA model group(n=40).After 10-day modeling,the AA model group was then further divided into the model group,the low dose NAC group,the median dose NAC group,and the high dose NAC group.The NAC groups were given 50,100,200 mg/kg NAC by gavage,respectively,for 16 d.Joint swelling degree and arthritis index was applied to evaluate the NAC therapeutic effect on AA rats;Immunohistochemical staining and Western blot were used to observe the expression of TLR2 protein in synovial tissue of joints.Serum levels of TNF⁃α,IL⁃1βand IL⁃10 were detected by ELISA.Results:Compared with the control group,the joint swelling and arthritis index in the model group were significant,and the joint swelling and arthritis index in the NAC treatment groups were significantly reduced(P<0.05,P<0.01).The expression of TLR2 protein in the syno⁃vial tissue of NAC treatment groups was decreased,inflammatory factors TNF⁃αand IL⁃1βwere decreased,and IL⁃10 was in⁃creased(P<0.01).Conclusion:NAC can improve the symptoms of arthritis in AA rats,and its mechanism may be related to inhi⁃bition of TLR2 and regulation of downstream inflammatory factor pathways in AA rats.

关 键 词：NAC TLR2 炎症因子 佐剂性关节炎 大鼠 

分 类 号：R965[医药卫生—药理学]